THE 3-DIMENSIONAL POINT-SPREAD FUNCTIONS OF A MICROSCOPE OBJECTIVE IN IMAGE AND OBJECT SPACE

被引：33

作者：

TAO, L

NICHOLSON, C

机构：

[1] Department of Physiology and Biophysics, New York University Medical School, New York, New York, 10016

来源：

JOURNAL OF MICROSCOPY | 1995年 / 178卷

关键词：

POINT SPREAD FUNCTION; OPTICAL TRANSFER FUNCTION; DEFOCUSED SYSTEM; 3-DIMENSIONAL IMAGE PROCESSING;

D O I：

10.1111/j.1365-2818.1995.tb03604.x

中图分类号：

TH742 [显微镜];

学科分类号：

摘要：

The three-dimensional point spread function (3-D PSF) of an optical system in image space is distinguished from the 3-D PSF in object space and the relation between the two 3-D PSFs is derived. By using this relation one 3-D PSF can be easily obtained from the other. The 3-D PSFs are given in a single integral expression, which can be computed numerically. The results of this study can be used in 3-D image processing for microscopy and have been applied to the analysis of the diffusion of fluorescent molecules in a 3-D porous medium.

引用

页码：267 / 271

页数：5

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