QUANTITATIVE MEASUREMENT OF EXTRARENAL RENIN MESSENGER-RNA BY POLYMERASE CHAIN-REACTION

被引：19

作者：

OKURA, T

KITAMI, Y

IWATA, T

HIWADA, K

机构：

[1] 2nd Department of Internal Medicine, Ehime University School of Medicine, Onsen-gun, Ehime

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 1991年 / 179卷 / 01期

关键词：

D O I：

10.1016/0006-291X(91)91328-A

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

We established a simplified method of the quantitative measurement of extra-renal renin messenger RNA using polymerase chain reaction system. We could detect the renin messenger RNA in the kidney, heart, aorta and adrenal gland from a single RNA sample obtained from a Wistar-Kyoto rat. In the kidney, heart, aorta and adrenal gland, the contents of renin messenger RNA were found to be 55.8 ± 17.8, 0.15 ± 0.05, 0.11 ± 0.03 and 0.16 ± 0.07 pgμg of total RNA (n=5, mean ± s.d.), respectively. The present method is very useful to study the extra-renal renin-angiotensin system. © 1991.

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页码：25 / 31

页数：7

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