LOTUS-JAPONICUS, AN AUTOGAMOUS, DIPLOID LEGUME SPECIES FOR CLASSICAL AND MOLECULAR-GENETICS

被引:458
作者
HANDBERG, K [1 ]
STOUGAARD, J [1 ]
机构
[1] AARHUS UNIV, DEPT MOLEC BIOL, GENE EXPRESS LAB, GUSTAV WIEDSVEJ 10, DK-8000 AARHUS, DENMARK
关键词
D O I
10.1111/j.1365-313X.1992.00487.x
中图分类号
Q94 [植物学];
学科分类号
071001 ;
摘要
In the Leguminosae plant family, few of the individual plant species have been used for plant molecular biology research. Among the species investigated no obvious representative 'model' legume has emerged. Here a member of the tribe Loteae, Lotus japonicus (Regel) Larsen is proposed as a candidate. L japonicus is a diploid, autogamous species, with a good seed set, and a generation time of approximately 3 months. The haploid genome consists of six chromosomes and the genome size was estimated to be relatively small (0.5 pg per haploid complement). L. japonicus is susceptible to Agrobacterium tumefaciens and transgenic plants can be regenerated after hygromycin or kanamycin selection. Tissue culture conditions and procedures for transformation and regeneration are described. Stable transformation is demonstrated by segregation of the hygromycin selectable marker after selfing of transgenic plants or test crosses. The possibility of mapping polymorphic DNA markers in inbred lines of L. japonicus is also discussed.
引用
收藏
页码:487 / 496
页数:10
相关论文
共 34 条
[1]  
Allen O. N., 1981, The Leguminosae. A source book of characteristics, uses and nodulation.
[2]  
BAJAJ YPS, 1990, BIOTECHNOLOGY AGR FO, V10
[3]   PHENOTYPIC ASSAY FOR EXCISION OF THE MAIZE CONTROLLING ELEMENT AC IN TOBACCO [J].
BAKER, B ;
COUPLAND, G ;
FEDOROFF, N ;
STARLINGER, P ;
SCHELL, J .
EMBO JOURNAL, 1987, 6 (06) :1547-1554
[4]  
Barker D. G., 1990, Plant Molecular Biology Reporter, V8, P40, DOI 10.1007/BF02668879
[5]   NUCLEAR-DNA AMOUNTS IN ANGIOSPERMS [J].
BENNETT, MD ;
SMITH, JB .
PHILOSOPHICAL TRANSACTIONS OF THE ROYAL SOCIETY B-BIOLOGICAL SCIENCES, 1976, 274 (933) :227-274
[6]  
Borsos O. S., 1973, ACTA BOT HUNG, V18, P49
[7]   CONTROL OF LEGHAEMOGLOBIN SYNTHESIS IN SNAKE BEANS [J].
BROUGHTON, WJ ;
DILWORTH, MJ .
BIOCHEMICAL JOURNAL, 1971, 125 (04) :1075-+
[8]  
BUBAR JS, 1985, CAN J BOT, V36, P65
[9]   SPECIES RELATIONSHIPS IN LOTUS-CORNICULATUS GROUP AS DETERMINED BY KARYOTYPE AND CYTOPHOTOMETRIC ANALYSES [J].
CHENG, RIJ ;
GRANT, WF .
CANADIAN JOURNAL OF GENETICS AND CYTOLOGY, 1973, 15 (01) :101-115
[10]   ISOLATION AND CHARACTERIZATION OF TRANSPOSON TN5-INDUCED SYMBIOTIC MUTANTS OF RHIZOBIUM-LOTI [J].
CHUA, KY ;
PANKHURST, CE ;
MACDONALD, PE ;
HOPCROFT, DH ;
JARVIS, BDW ;
SCOTT, DB .
JOURNAL OF BACTERIOLOGY, 1985, 162 (01) :335-343