INVIVO PROMOTER ACTIVITY OF THE SYNTHETIC PRIBNOW BOX

被引：6

作者：

KOROLEVA, ON

DRUTSA, VL

机构：

[1] A.N. Belozersky Laboratory of Molecular Biology and Bioorganic Chemistry, Moscow State University, Leninskiye Gory

来源：

FEBS LETTERS | 1991年 / 278卷 / 02期

关键词：

RECOMBINANT PLASMID; GAL OPERON; PRIBNOW BOX; PCR; S1-MAPPING;

D O I：

10.1016/0014-5793(91)80118-M

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The synthetic polydeoxyribonucleotides TGCA(TTATAATGCA)1-4, containing the consensus Pribnow box, were inserted upstream of the promoterless gal operon in in a specially constructed plasmid. Using the MacConkey agar indicator technique it has been shown that these inserts are able to induce an efficient in vivo expression of the gal operon.

引用

页码：207 / 210

页数：4

共 14 条

[1] CHARACTERIZATION OF 2 MUTATIONS IN THE ESCHERICHIA-COLI GALE GENE INACTIVATING THE 2ND GALACTOSE OPERATOR AND COMPARATIVE STUDIES OF REPRESSOR BINDING [J].

FRITZ, HJ ;

BICKNASE, H ;

GLEUMES, B ;

HEIBACH, C ;

ROSAHL, S ;

EHRING, R .

EMBO JOURNAL, 1983, 2 (12) :2129-2135

[2] ANALYSIS OF ESCHERICHIA-COLI PROMOTER SEQUENCES [J].

HARLEY, CB ;

REYNOLDS, RP .

NUCLEIC ACIDS RESEARCH, 1987, 15 (05) :2343-2361

[3]

HORWITZ MSZ, 1988, J BIOL CHEM, V263, P14724

[4] TRANSCRIPTION INVIVO DIRECTED BY CONSENSUS SEQUENCES OF ESCHERICHIA-COLI PROMOTERS - THEIR CONTEXT HEAVILY AFFECTS EFFICIENCIES AND START SITES [J].

JACQUET, MA ;

REISS, C .

NUCLEIC ACIDS RESEARCH, 1990, 18 (05) :1137-1143

[5] INVIVO GENE-EXPRESSION DIRECTED BY SYNTHETIC PROMOTER CONSTRUCTIONS RESTRICTED TO THE -10 AND -35 CONSENSUS HEXAMERS OF ESCHERICHIA-COLI [J].

JACQUET, MA ;

EHRLICH, R ;

REISS, C .

NUCLEIC ACIDS RESEARCH, 1989, 17 (08) :2933-2945

[6]

KOROLEVA O N, 1985, Molekulyarnaya Biologiya (Moscow), V19, P516

[7]

KOROLEVA ON, 1984, MOLEKULARNAYA BIOL, V10, P148

[8]

Maniatis T., 1982, MOL CLONING

[9] NUCLEOTIDE CHEMISTRY .4. SYNTHESIS OF DEOXYOLIGONUCLEOTIDES ON A POLYMER SUPPORT [J].

MATTEUCCI, MD ;

CARUTHERS, MH .

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 1981, 103 (11) :3185-3191

[10]

Maxam A M, 1980, Methods Enzymol, V65, P499

← 1 2 →