BINARY BUFFERS FOR INDIRECT ABSORPTION DETECTION IN CAPILLARY ZONE ELECTROPHORESIS

被引：39

作者：

WANG, T ^{[1
]}

HARTWICK, RA ^{[1
]}

机构：

[1] SUNY,DEPT CHEM BINGHAMTON,BINGHAMTON,NY 13902

来源：

JOURNAL OF CHROMATOGRAPHY | 1992年 / 589卷 / 1-2期

关键词：

D O I：

10.1016/0021-9673(92)80037-U

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

The displacement processes in binary buffers (two acids and one base) were studied theoretically and experimentally. An equation that correlates the concentration change of buffer ingredients in the sample zone with their electrophoretic mobility was derived. According to the equation, when the mobility of a sample is between those of the two buffer components, the sample will mainly displace the species to which its mobility is closest. This displacement is termed mobility-selective displacement. Several binary buffers with different mobilities were studied, and the experimental results agreed semi-quantitatively with the theory.

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页码：307 / 313

页数：7

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