HUMAN-ANTIBODY FRAGMENTS SPECIFIC FOR HUMAN BLOOD-GROUPS ANTIGENS FROM A PHAGE DISPLAY LIBRARY

被引：188

作者：

MARKS, JD

OUWEHAND, WH

BYE, JM

FINNERN, R

GORICK, BD

VOAK, D

THORPE, SJ

HUGHESJONES, NC

WINTER, G

机构：

[1] UNIV CAMBRIDGE,E ANGLIAN BLOOD TRANSFUS CTR,DIV TRANSFUS MED,LONG RD,CAMBRIDGE CB2 3PT,ENGLAND

[2] MRC,CTR PROT ENGN,CAMBRIDGE CB2 2QH,ENGLAND

[3] NATL INST BIOL STAND & CONTROLS,DIV HAEMATOL,POTTERS BAR EN6 3Q6,ENGLAND

[4] MRC,IMMUNOPATHOL UNIT,CAMBRIDGE CB2 2QH,ENGLAND

来源：

BIO-TECHNOLOGY | 1993年 / 11卷 / 10期

关键词：

D O I：

10.1038/nbt1093-1145

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

We have isolated human antibody fragments with binding specificities against the blood group antigens of the ABO and I blood group systems (B and HI), of the Rh system (D and E) and of the Kell system (Kp(b)), by selecting a phage-antibody library on human red cells. The fragments, expressed in bacteria, were antigen-specific and effective in assays including agglutination and immunohistochemistry. Selection of phage-antibody libraries using intact cells seems to offer a promising alternative to hybridoma technology for the production of antibodies against cell surface molecules.

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收藏

页码：1145 / 1149

页数：5

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