2,2'-DITHIOBIS(1-AMINO-4,5-DIMETHOXYBENZENE) AS A HIGHLY SENSITIVE, SELECTIVE AND STABLE FLUORESCENCE DERIVATIZATION REAGENT FOR AROMATIC-ALDEHYDES IN LIQUID-CHROMATOGRAPHY

被引：6

作者：

HARA, S ^{[1
]}

NAKAMURA, M ^{[1
]}

SAKAI, F ^{[1
]}

NOHTA, H ^{[1
]}

OHKURA, Y ^{[1
]}

YAMAGUCHI, M ^{[1
]}

机构：

[1] KYUSHU UNIV 62,FAC PHARMACEUT SCI,HIGASHI KU,FUKUOKA 812,JAPAN

来源：

ANALYTICA CHIMICA ACTA | 1994年 / 291卷 / 1-2期

关键词：

FLUOROMETRY; LIQUID CHROMATOGRAPHY; ALDEHYDES; DITHIOBIS(1-AMINO-4,5-DIMETHOXYBENZENE);

D O I：

10.1016/0003-2670(94)85142-5

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

2,2'-Dithiobis(1-amino-4,5-dimethoxybenz was synthesized as a highly sensitive, selective and stable fluorescence derivatization reagent for aromatic aldehydes in liquid chromatography. The reagent reacts selectively with aromatic aldehydes in acidic media in the presence of tri-n-butylphosphine, sodium sulphite and disodium hydrogenphosphite. The reaction was complete within 60 min at 37 degrees C. The fluorescent products from benzaldehyde and 4-hydroxybenzaldehyde are shown to be 2-phenyl-5,6-dimethoxybenzothiazole and 2-(4-hydroxyphenyl)-5,6-dimethoxybenzothiazole, respectively. The fluorescent derivatives of aromatic aldehydes can be separated by reversed-phase chromatography and their detection limits (signal-to-noise ratio = 3) are 8-20 fmol on-column.

引用

页码：189 / 195

页数：7