SPECIFIC RECEPTORS FOR ENDOTHELIN-3 IN CULTURED BOVINE ENDOTHELIAL-CELLS AND ITS CELLULAR MECHANISM OF ACTION

被引:136
作者
EMORI, T [1 ]
HIRATA, Y [1 ]
MARUMO, F [1 ]
机构
[1] TOKYO MED & DENT UNIV,DEPT INTERNAL MED 2,DIV ENDOCRINE,1-5-45 YUSHIMA,BUNKYO KU,TOKYO 113,JAPAN
关键词
Endothelial cell; Endothelin-3; Vasodilatation;
D O I
10.1016/0014-5793(90)81388-5
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Among three endothelin (ET) isopeptides, ET-3 shows the most potent initial depressor response through the endothelium-dependent mechanism. We studied the presence of specific binding sites for ET-3 in cultured bovine endothelial cells (EC) and its cellular mechanism of action. Binding studies revealed the presence of two distinct subclasses of ET-3 receptors with high and low affinities. ET-3 dose-dependently (10-10-10-7 M) increased both intracellular Ca2+ levels ([Ca-2]) and inositol trisphosphate (IP3) formation. The ET-3-induced increase in [Ca2+]i was not affected by either removal of extracellular Ca2+ or Ca2+-channel blockers. These data suggest that ET-3 induces phosphoinositide breakdown and increase in [Ca2+]i in ECs, possibly resulting from intracellular Ca2+ mobilization, thereby leading to vasodilatation. © 1990.
引用
收藏
页码:261 / 264
页数:4
相关论文
共 12 条
[1]   HIGH AND LOW AFFINITY BINDING-SITES FOR ENDOTHELIN ON CULTURED RAT GLOMERULAR MESANGIAL CELLS [J].
BADR, KF ;
MUNGER, KA ;
SUGIURA, M ;
SNAJDAR, RM ;
SCHWARTZBERG, M ;
INAGAMI, T .
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 1989, 161 (02) :776-781
[2]  
DENUCCI G, 1988, P NATL ACAD SCI USA, V85, P9797
[3]   MEDIATORS PRODUCED BY THE ENDOTHELIAL-CELL [J].
GRYGLEWSKI, RJ ;
BOTTING, RM ;
VANE, JR .
HYPERTENSION, 1988, 12 (06) :530-548
[4]  
GRYKIEWICZ G, 1985, J BIOL CHEM, V260, P3440
[5]   CELLULAR MECHANISM OF ACTION BY A NOVEL VASOCONSTRICTOR ENDOTHELIN IN CULTURED RAT VASCULAR SMOOTH-MUSCLE CELLS [J].
HIRATA, Y ;
YOSHIMI, H ;
TAKATA, S ;
WATANABE, TX ;
KUMAGAI, S ;
NAKAJIMA, K ;
SAKAKIBARA, S .
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 1988, 154 (03) :868-875
[6]   THE HUMAN ENDOTHELIN FAMILY - 3 STRUCTURALLY AND PHARMACOLOGICALLY DISTINCT ISOPEPTIDES PREDICTED BY 3 SEPARATE GENES [J].
INOUE, A ;
YANAGISAWA, M ;
KIMURA, S ;
KASUYA, Y ;
MIYAUCHI, T ;
GOTO, K ;
MASAKI, T .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1989, 86 (08) :2863-2867
[7]   2 DIFFERENT FORMS OF ENDOTHELIN RECEPTORS IN RAT LUNG [J].
MASUDA, Y ;
MIYAZAKI, H ;
KONDOH, M ;
WATANABE, H ;
YANAGISAWA, M ;
MASAKI, T ;
MURAKAMI, K .
FEBS LETTERS, 1989, 257 (02) :208-210
[8]   THE ROLE OF PROTEIN KINASE-C IN CELL-SURFACE SIGNAL TRANSDUCTION AND TUMOR PROMOTION [J].
NISHIZUKA, Y .
NATURE, 1984, 308 (5961) :693-698
[9]   ENDOTHELIN STIMULATES PHOSPHOLIPASE-C IN CULTURED VASCULAR SMOOTH-MUSCLE CELLS [J].
RESINK, TJ ;
SCOTTBURDEN, T ;
BUHLER, FR .
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 1988, 157 (03) :1360-1368
[10]   RAT ENDOTHELIN IS A VASODILATOR IN THE ISOLATED PERFUSED MESENTERY OF THE RAT [J].
WARNER, TD ;
DENUCCI, G ;
VANE, JR .
EUROPEAN JOURNAL OF PHARMACOLOGY, 1989, 159 (03) :325-326