ISOLATION OF A MAMMALIAN SEQUENCE CAPABLE OF CONFERRING CELL-CYCLE REGULATION TO A HETEROLOGOUS GENE

被引：41

作者：

ARTISHEVSKY, A ^{[1
]}

GRAFSKY, A ^{[1
]}

LEE, AS ^{[1
]}

机构：

[1] UNIV SO CALIF, SCH MED, DEPT BIOCHEM, LOS ANGELES, CA 90033 USA

来源：

SCIENCE | 1985年 / 230卷 / 4729期

关键词：

D O I：

10.1126/science.4059922

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

A hybrid gene containing the 5'' sequence of a hamster histone H3 gene and the coding sequence of the bacterial neomycin-resisance gene (neo) was constructed. Upon transfection into the hamster fibroblasts cell line K12, the hybrid gene exhibited cell cycle-dependent regulation, as evidence by the maximal accumulation of the neo transcripts during synthesis of DNA in the cell cycle. In addition, cells arrested in the prereplicative phase, as a consequence of the K12 temperature-sensitive mutation, produced significantly less neo messenger RNA.

引用

页码：1061 / 1063

页数：3

共 18 条

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