SEQUENCE AND TRANSCRIPT ANALYSIS OF THE NCO2.5 OGURA-SPECIFIC FRAGMENT CORRELATED WITH CYTOPLASMIC MALE-STERILITY IN BRASSICA CYBRIDS

被引：160

作者：

BONHOMME, S ^{[1
]}

BUDAR, F ^{[1
]}

LANCELIN, D ^{[1
]}

SMALL, I ^{[1
]}

DEFRANCE, MC ^{[1
]}

PELLETIER, G ^{[1
]}

机构：

[1] INRA, CTR VERSAILLES, BIOL CELLULAIRE LAB, F-78026 VERSAILLES, FRANCE

来源：

MOLECULAR AND GENERAL GENETICS | 1992年 / 235卷 / 2-3期

关键词：

BRASSICA; MITOCHONDRIAL DNA (MTDNA); OGURA; ORF138; ORF158;

D O I：

10.1007/BF00279379

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Sequence analysis of the Ogura-specific mitochondrial DNA (mtDNA) fragment isolated previously from Brassica cybrids carrying Ogura cytoplasmic male sterility (cms) revealed a tRNA(fMet) sequence, a putative 138 amino acid open reading frame (orf138), and a 158 amino acid ORF (orf158) previously observed in mitochondrial genomes from several other plant species. Transcription mapping showed that both ORFs are present on a 1.4 kb cms-specific transcript. The orf158 sequence is also transcribed in fertile plants on a different mRNA, and thus is unlikely to be related to cms. On the other hand, fertile revertant plants lack transcripts of the orf138 sequence, whose possible role in the mechanism of Ogura cms is discussed.

引用

页码：340 / 348

页数：9

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