FORMATION OF F-2-ISOPROSTANES DURING AORTIC ENDOTHELIAL CELL-MEDIATED OXIDATION OF LOW-DENSITY-LIPOPROTEIN

被引：58

作者：

GOPAUL, NK

NOUROOZZADEH, J

MALLET, AI

ANGGARD, EE

机构：

[1] UNIV LONDON UNIV COLL,DEPT MED,DIV CLIN PHARMACOL & TOXICOL,LONDON WC1E 6JJ,ENGLAND

[2] UNITED MED & DENT SCH GUYS & ST THOMASS HOSP,ST JOHNS INST DERMATOL,LONDON SE1 7EH,ENGLAND

来源：

FEBS LETTERS | 1994年 / 348卷 / 03期

关键词：

F-2-ISOPROSTANE; LDL; CELL-MEDIATED OXIDATION; LIPID PEROXIDATION; ATHEROSCLEROSIS;

D O I：

10.1016/0014-5793(94)00628-8

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

We investigated the formation of F-2-isoprostanes produced by non-enzymatic peroxidation of arachidonic acid during rabbit aortic endothelial cell-mediated oxidation of low density lipoprotein (LDL). Free and total (sum of free and esterified) levels of F-2-isoprostanes were measured using a solid-phase extraction procedure and gas chromatography-mass spectrometry. Free levels of F-2-isoprostanes in native LDL were 0.06 +/- 0.03 ng/mg protein (n = 4), whereas total levels were 0.28 +/- 0.09 ng/mg protein (n 4). Both free and total levels of the isoprostanes were found to increase during the oxidation. 8-epi-PGF(2), was the major isoprostane formed (free and total concentrations after 24 h, 2.50 +/- 0.24 and 6.42 +/- 1.36 ng/mg protein (n = 4), respectively). The release of F-2-isoprostanes during aortic endothelial cell-induced oxidation of LDL could be a contributory factor in the development of atherosclerosis.

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页码：297 / 300

页数：4

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