PURIFICATION AND SOME PROPERTIES OF A XYLANASE FROM ASPERGILLUS-SYDOWII-MG49

被引：22

作者：

GHOSH, M ^{[1
]}

NANDA, G ^{[1
]}

机构：

[1] BOSE INST,DEPT MICROBIOL,CALCUTTA 700054,W BENGAL,INDIA

来源：

APPLIED AND ENVIRONMENTAL MICROBIOLOGY | 1994年 / 60卷 / 12期

关键词：

D O I：

10.1128/AEM.60.12.4620-4623.1994

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Aspergillus sydowii MG49 produces a 30-kDa exosplitting xylobiohydrolase during growth on xylan. A specific chemical modification and substrate protection analysis of purified xylanase provided evidence that tryptophan and carboxy and amino groups are present at the catalytic site of this enzyme. Thermal inactivation of the xylanase occurs because of irreversible polymolecular aggregation,,which is slower in the presence of glycerol.

引用

页码：4620 / 4623

页数：4

共 23 条

[1] ESSENTIAL CARBOXY GROUPS IN XYLANASE-A [J].

BRAY, MR ;

CLARKE, AJ .

BIOCHEMICAL JOURNAL, 1990, 270 (01) :91-96

[2] USE OF A MODIFIED CUPRIC ACETATE METHOD FOR THE DETECTION AND QUANTITATION OF XYLANOLYTIC ACTIVITIES - A COMPARATIVE-STUDY WITH THE CONGO RED METHOD [J].

CAPALASH, N ;

SHARMA, P ;

GUPTA, KG .

LETTERS IN APPLIED MICROBIOLOGY, 1990, 10 (03) :151-154

[3] MECHANISMS OF THERMOINACTIVATION OF ENDOGLUCANASE-I FROM TRICHODERMA-REESEI QM-9414 [J].

DOMINGUEZ, JM ;

ACEBAL, C ;

JIMENEZ, J ;

DELAMATA, I ;

MACARRON, R ;

CASTILLON, MP .

BIOCHEMICAL JOURNAL, 1992, 287 :583-588

[4] ASPERGILLUS-SYDOWII MG-49 IS A STRONG PRODUCER OF THERMOSTABLE XYLANOLYTIC ENZYMES [J].

GHOSH, M ;

DAS, A ;

MISHRA, AK ;

NANDA, G .

ENZYME AND MICROBIAL TECHNOLOGY, 1993, 15 (08) :703-709

[5] THERMOSTABILITY OF BETA-XYLOSIDASE FROM ASPERGILLUS-SYDOWII MG49 [J].

GHOSH, M ;

NANDA, G .

FEBS LETTERS, 1993, 330 (03) :275-278

[6] STUDIES ON XYLAN DEGRADING ENZYMES .1. PURIFICATION AND CHARACTERIZATION OF ENDO-1,4-BETA-XYLANASE FROM ASPERGILLUS-NIGER STR-14 [J].

GORBACHEVA, IV ;

RODIONOVA, NA .

BIOCHIMICA ET BIOPHYSICA ACTA, 1977, 484 (01) :79-93

[7]

HASS H, 1992, BIOCHIM BIOPHYS ACTA, V1117, P279

[8] NEW FAMILIES IN THE CLASSIFICATION OF GLYCOSYL HYDROLASES BASED ON AMINO-ACID-SEQUENCE SIMILARITIES [J].

HENRISSAT, B ;

BAIROCH, A .

BIOCHEMICAL JOURNAL, 1993, 293 :781-788

[9] CHARACTERIZATION AND SEQUENCE OF A THERMOMONOSPORA-FUSCA XYLANASE [J].

IRWIN, D ;

JUNG, ED ;

WILSON, DB .

APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 1994, 60 (03) :763-770

[10] CHEMICAL MODIFICATION OF A XYLANASE FROM A THERMOTOLERANT STREPTOMYCES - EVIDENCE FOR ESSENTIAL TRYPTOPHAN AND CYSTEINE RESIDUES AT THE ACTIVE-SITE [J].

KESKAR, SS ;

SRINIVASAN, MC ;

DESHPANDE, VV .

BIOCHEMICAL JOURNAL, 1989, 261 (01) :49-55

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