HYDROPHOBIC INTERACTION CHROMATOGRAPHY OF CHROMOBACTERIUM-VISCOSUM LIPASE

被引：18

作者：

QUEIROZ, JA

GARCIA, FAP

CABRAL, JMS

机构：

[1] INST SUPER TECN,ENGN BIOQUIM LAB,P-1000 LISBON,PORTUGAL

[2] UNIV BEIRA INTERIOR,DEPT QUIM,P-6200 COVILHA,PORTUGAL

[3] UNIV COIMBRA,DEPT ENGN QUIM,P-3000 COIMBRA,PORTUGAL

来源：

JOURNAL OF CHROMATOGRAPHY A | 1995年 / 707卷 / 02期

关键词：

D O I：

10.1016/0021-9673(95)00134-9

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

The influence of mobile phase composition on the chromatographic behaviour of Chromobacterium viscosum lipase was studied by using an epoxy-activated spacer arm as a ligand in hydrophobic interaction chromatography. The retention of lipase depends on the salt used and increased with ionic strength, indicating that the interaction of lipase with the stationary phase is of a hydrophobic nature. Using 20% (w/v) ammonium sulphate in the eluent a total retention of lipase on the column was obtained and by washing with 10 mM phosphate buffer a recovery of 79% protein and 89% lipolytic activity were achieved.

引用

页码：137 / 142

页数：6

共 12 条

[1]

BRADFORD MM, 1976, ANAL BIOCHEM, V72, P248, DOI 10.1016/0003-2697(76)90527-3

[2] SOME GENERAL ASPECTS OF HYDROPHOBIC INTERACTION CHROMATOGRAPHY [J].