CLONED ORIGIN OF DNA-REPLICATION IN C-MYC GENE CAN FUNCTION AND BE TRANSMITTED IN TRANSGENIC MICE IN AN EPISOMAL STATE

被引:28
作者
SUDO, K
OGATA, M
SATO, Y
IGUCHIARIGA, SMM
ARIGA, H
机构
[1] HOKKAIDO UNIV,FAC PHARMACEUT SCI,KITA 12 JOU,NISHI 6 CHOME,KITA KU,SAPPORO,HOKKAIDO 060,JAPAN
[2] UNIV TOKYO,INST MED SCI,MINATO KU,TOKYO 108,JAPAN
[3] DAIICHI SEIYAKU CO LTD,RES INST,EDOGAWA KU,TOKYO 134,JAPAN
关键词
D O I
10.1093/nar/18.18.5425
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
The c-myc protein has recently been shown to interact with a region possessing putative origin of DNA replication and enhancer activities located 2 kb upstream of the c-myc gene itself. Transgenic mice were obtained by injecting constructs containing this region, termed pmyc(H-P), into fertilized mouse eggs. The transgenic elements were capable of efficient replication in all mouse tissues examined and were maintained in an episomal state even in highly differentiated cells. Moreover, pmyc(H-P) was transmittable to the progeny throughout several generations, which suggests that the fragment derived from the region upstream of the c-myc gene possesses sequences necessary for partition, stability and DNA replication of the plasmid in the cells. In addition, we have shown that the plasmid might be captured only by eggs, not by sperm. © 1990 Oxford University Press.
引用
收藏
页码:5425 / 5432
页数:8
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