THE CLONING AND SEQUENCE-ANALYSIS OF THE RAT SEROTONIN-1A RECEPTOR GENE

被引：13

作者：

FUJIWARA, Y ^{[1
]}

NELSON, DL ^{[1
]}

KASHIHARA, K ^{[1
]}

VARGA, E ^{[1
]}

ROESKE, WR ^{[1
]}

YAMAMURA, HI ^{[1
]}

机构：

[1] UNIV ARIZONA, COLL PHARM, DEPT PHARMACOL & TOXICOL, TUCSON, AZ 85724 USA

来源：

LIFE SCIENCES | 1990年 / 47卷 / 22期

基金：

美国国家卫生研究院;

关键词：

D O I：

10.1016/0024-3205(90)90445-W

中图分类号：

R-3 [医学研究方法]; R3 [基础医学];

学科分类号：

1001 ;

摘要：

Using a probe derived from the human 5-HT1A receptor gene (G-21), a strongly hybridizable clone was identified from a rat genomic DNA library. This clone (G21Y2) had an overall deduced amino acid sequence that was 88% homologous with that of the human 5-HT1A receptor. There was only an 18% and 19% amino acid homology with the rat 5-HT1C and 5-HT2 receptors, respectively. The G21Y2 appears to be an excellent candidate for the rat 5-HT1A receptor gene. As this manuscript was being prepared a report by Albert et al. (13) was published in which a probe to the hamster β2-adrenergic receptor was used to identify a rat genomic (Clone D) that demonstrated a sequence that was similar to G21Y2. © 1990.

引用

页码：PL127 / PL132

页数：6

共 13 条

[1]

ALBERT PR, 1990, J BIOL CHEM, V265, P5825

[2] BUFFER GRADIENT GELS AND S-35 LABEL AS AN AID TO RAPID DNA-SEQUENCE DETERMINATION [J].

BIGGIN, MD ;

GIBSON, TJ ;

HONG, GF .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA-BIOLOGICAL SCIENCES, 1983, 80 (13) :3963-3965

[3] THE GENOMIC CLONE G-21 WHICH RESEMBLES A BETA-ADRENERGIC-RECEPTOR SEQUENCE ENCODES THE 5-HT1A RECEPTOR [J].

FARGIN, A ;

RAYMOND, JR ;

LOHSE, MJ ;

KOBILKA, BK ;

CARON, MG ;

LEFKOWITZ, RJ .

NATURE, 1988, 335 (6188) :358-360

[4]

FARGIN A, 1989, J BIOL CHEM, V264, P14848

[5]

FRAZER A, 1990, ANNU REV PHARMACOL, V30, P307

[6] MOLECULAR-BIOLOGY OF 5-HT RECEPTORS [J].

HARTIG, PR .

TRENDS IN PHARMACOLOGICAL SCIENCES, 1989, 10 (02) :64-69

[7] MOLECULAR CHARACTERIZATION OF A FUNCTIONAL CDNA-ENCODING THE SEROTONIN 1C RECEPTOR [J].

JULIUS, D ;

MACDERMOTT, AB ;

AXEL, R ;

JESSELL, TM .

SCIENCE, 1988, 241 (4865) :558-564

[8] AN INTRONLESS GENE ENCODING A POTENTIAL MEMBER OF THE FAMILY OF RECEPTORS COUPLED TO GUANINE-NUCLEOTIDE REGULATORY PROTEINS [J].

KOBILKA, BK ;

FRIELLE, T ;

COLLINS, S ;

YANGFENG, T ;

KOBILKA, TS ;

FRANCKE, U ;

LEFKOWITZ, RJ ;

CARON, MG .

NATURE, 1987, 329 (6134) :75-79

[9] STRUCTURE AND FUNCTIONAL EXPRESSION OF CLONED RAT SEROTONIN 5HT-2 RECEPTOR [J].

PRITCHETT, DB ;

BACH, AWJ ;

WOZNY, M ;

TALEB, O ;

DALTOSO, R ;

SHIH, JC ;

SEEBURG, PH .

EMBO JOURNAL, 1988, 7 (13) :4135-4140

[10] DNA SEQUENCING WITH CHAIN-TERMINATING INHIBITORS [J].

SANGER, F ;

NICKLEN, S ;

COULSON, AR .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1977, 74 (12) :5463-5467

← 1 2 →