MAPPING OF THE PROTEIN IMPORT MACHINERY IN THE MITOCHONDRIAL OUTER-MEMBRANE BY CROSS-LINKING OF TRANSLOCATION INTERMEDIATES

被引:153
作者
SOLLNER, T
RASSOW, J
WIEDMANN, M
SCHLOSSMANN, J
KEIL, P
NEUPERT, W
PFANNER, N
机构
[1] UNIV MUNICH, INST PHYSIOL CHEM, GOETHESTR 33, W-8000 MUNICH 2, GERMANY
[2] AKAD WISSENSCH DDR, ZENT INST MOLEK BIOL, O-1115 BERLIN, GERMANY
关键词
D O I
10.1038/355084a0
中图分类号
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号
07 ; 0710 ; 09 ;
摘要
MITOCHONDRIA Contain a complex machinery for the import of nuclear-encoded proteins 1,2. Receptor proteins exposed on the outer membrane surface are required for the specific binding of precursor proteins to mitochondria, either by binding of cytosolic signal recognition factors or by direct recognition of the precursor polypeptides 1-5. Subsequently, the precursors are inserted into the outer membrane at the general insertion site GIP (general insertion protein) 6-10. Here we report the analysis of receptors and GIP by crosslinking of translocation intermediates and by coimmunoprecipitation. Surface-accumulated precursors were crosslinked to the receptors MOM19 and MOM72, suggesting a direct interaction of preproteins with surface receptors. We identified three novel mitochondrial outer membrane proteins, MOM7, MOM8, and MOM30 that, together with the previously identified MOM38, seem to form the GIP site and are present in the mitochondrial receptor com lex.
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页码:84 / 87
页数:4
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