A NEW NUCLEAR SUPPRESSOR SYSTEM FOR A MITOCHONDRIAL RNA-POLYMERASE MUTANT IDENTIFIES AN UNUSUAL ZINC-FINGER PROTEIN AND A POLYGLUTAMINE DOMAIN PROTEIN IN SACCHAROMYCES-CEREVISIAE

被引:29
作者
BROHL, S [1 ]
LISOWSKY, T [1 ]
RIEMEN, G [1 ]
MICHAELIS, G [1 ]
机构
[1] UNIV DUSSELDORF,INST BOT,D-40225 DUSSELDORF,GERMANY
关键词
TRANSCRIPTION FACTORS; MITOCHONDRIAL RNA POLYMERASE; ZINC-FINGER PROTEIN; GLUTAMINE DOMAIN; SACCHAROMYCES CEREVISIAE;
D O I
10.1002/yea.320100604
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
A yeast strain with a point mutation in the nuclear gene for the core subunit of mitochondrial RNA polymerase was used to isolate new extragenic suppressors. Spontaneously occurring phenotypical revertants were analysed by crosses with the wild-type and tetrad dissection. One of the new nuclear suppressor mutants was characterized by temperature-sensitive growth on non-fermentable carbon sources. This mutant was transformed with a genomic yeast library. Two independent types of DNA clones were isolated which both complemented the temperature sensitive defect. Subcloning and DNA sequencing identified two novel yeast genes which code for proteins with the characteristic features of transcription factors. Both factors exhibit highly structured protein domains consisting of runs and clusters of asparagine and glutamine residues. One of the proteins contains in addition zinc-finger domains of the C2H2-type. Therefore the genes are proposed to be named AZFI (asparagine-rich zinc-finger protein) and PGD1 (polyglutamine domain protein). Gene disruption of both reading frames has no detectable influence on the vegetative growth on complete glucose or glycerol media, indicating that the genes may act as high copy number suppressors of the mutant defect. Additional transformation experiments showed that AZFI is also an efficient suppressor for the original defect in the core subunit of mitochondrial RNA polymerase. The DNA sequences for the AZFI and PGD1 genes were submitted to the EMBL data base (Accession Numbers: Z26253 and Z26254).
引用
收藏
页码:719 / 731
页数:13
相关论文
共 42 条
[1]  
ATTARDI G, 1988, ANNU REV CELL BIOL, V4, P289, DOI 10.1146/annurev.cb.04.110188.001445
[2]   STUDIES ON THE STRUCTURE, EXPRESSION AND FUNCTION OF THE YEAST REGULATORY GENE PHO2 [J].
BERBEN, G ;
LEGRAIN, M ;
HILGER, F .
GENE, 1988, 66 (02) :307-312
[3]   ASSOCIATION OF CHARGE CLUSTERS WITH FUNCTIONAL DOMAINS OF CELLULAR TRANSCRIPTION FACTORS [J].
BRENDEL, V ;
KARLIN, S .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1989, 86 (15) :5698-5702
[4]   THE DAL81 GENE-PRODUCT IS REQUIRED FOR INDUCED EXPRESSION OF 2 DIFFERENTLY REGULATED NITROGEN CATABOLIC GENES IN SACCHAROMYCES-CEREVISIAE [J].
BRICMONT, PA ;
DAUGHERTY, JR ;
COOPER, TG .
MOLECULAR AND CELLULAR BIOLOGY, 1991, 11 (02) :1161-1166
[5]  
BROACH JR, 1979, GENE, V8, P121, DOI 10.1016/0378-1119(79)90012-X
[6]   SYNERGISTIC ACTIVATION BY THE GLUTAMINE-RICH DOMAINS OF HUMAN TRANSCRIPTION FACTOR SP1 [J].
COUREY, AJ ;
HOLTZMAN, DA ;
JACKSON, SP ;
TJIAN, R .
CELL, 1989, 59 (05) :827-836
[7]   ANALYSIS OF SP1 INVIVO REVEALS MULTIPLE TRANSCRIPTIONAL DOMAINS, INCLUDING A NOVEL GLUTAMINE-RICH ACTIVATION MOTIF [J].
COUREY, AJ ;
TJIAN, R .
CELL, 1988, 55 (05) :887-898
[8]   A CLOSE RELATIVE OF THE NUCLEAR, CHROMOSOMAL HIGH-MOBILITY GROUP PROTEIN HMG1 IN YEAST MITOCHONDRIA [J].
DIFFLEY, JFX ;
STILLMAN, B .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1991, 88 (17) :7864-7868
[9]   STUDIES ON TRANSFORMATION OF ESCHERICHIA-COLI WITH PLASMIDS [J].
HANAHAN, D .
JOURNAL OF MOLECULAR BIOLOGY, 1983, 166 (04) :557-580
[10]   MITOCHONDRIAL PROTEIN IMPORT [J].
HARTL, FU ;
PFANNER, N ;
NICHOLSON, DW ;
NEUPERT, W .
BIOCHIMICA ET BIOPHYSICA ACTA, 1989, 988 (01) :1-45