THE MAP KINASE CASCADE IS NOT ESSENTIAL FOR TRANSCRIPTIONAL STIMULATION OF OSMOLYTE TRANSPORTER GENES

被引：60

作者：

KWON, HM ^{[1
]}

ITOH, T ^{[1
]}

RIM, JS ^{[1
]}

HANDLER, JS ^{[1
]}

机构：

[1] OSAKA UNIV,SCH MED,DEPT MED 1,SUITA,OSAKA,JAPAN

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 1995年 / 213卷 / 03期

关键词：

D O I：

10.1006/bbrc.1995.2224

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Kidney derived MDCK cells are protected from the stress of hypertonicity by accumulating compatible osmolytes. Accumulation of the compatible osmolytes myo-inositol and betaine is driven by hypertonicity-induced stimulation of transcription of the genes coding for the myoinositol cotransporter and the betaine cotransporter. We tested the importance of the mitogen-activated protein kinase pathway in transcriptional activation of the genes for the two osmolytes cotransporters because this kinase pathway is rapidly activated when cells are exposed to hypertonicity and a mitogen-activated protein kinase pathway is essential for the osmo-protective transcriptional response of yeast to hypertonicity. Eliminating the activation of mitogen-activated protein kinase did not block the hypertonicity induced increase in accumulation of osmolyte transporter mRNA. (C) 1995 Academic Press, Inc.

引用

页码：975 / 979

页数：5

共 14 条

[1] GPD1, WHICH ENCODES GLYCEROL-3-PHOSPHATE DEHYDROGENASE, IS ESSENTIAL FOR GROWTH UNDER OSMOTIC-STRESS IN SACCHAROMYCES-CEREVISIAE, AND ITS EXPRESSION IS REGULATED BY THE HIGH-OSMOLARITY GLYCEROL RESPONSE PATHWAY [J].