FERMENTATION OF SUGARS IN ORANGE PEEL HYDROLYSATES TO ETHANOL BY RECOMBINANT ESCHERICHIA-COLI KO11

被引：34

作者：

GROHMANN, K ^{[1
]}

CAMERON, RG ^{[1
]}

BUSLIG, BS ^{[1
]}

机构：

[1] FLORIDA DEPT CITRUS, WINTER HAVEN, FL 33883 USA

来源：

APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY | 1995年 / 51-2卷

关键词：

ORANGE PEEL; ENZYMATIC HYDROLYSIS; ETHANOL; GALACTURONIC ACID FERMENTATION; RECOMBINANT ESCHERICHIA-COLI B;

D O I：

10.1007/BF02933445

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The conversion of monosaccharides in orange peel hydrolysates to ethanol by recombinant Escherichia coli KO11 has been investigated in pH-controlled batch fermentations at 32 and 37 degrees C. pH values and concentration of peel hydrolysate were varied to determine approximate optimal conditions and limitations of these fermentations. Very high yields of ethanol were achieved by this microorganism at reasonable ethanol concentrations (28-48 g/L). The pH range between 5.8 and 6.2 appears to be optimal. The microorganism can convert all major monosaccharides in orange peel hydrolysates to ethanol and to smaller amounts of acetic and lactic acids. Acetic acid is coproduced in equimolar amounts with ethanol by catabolism of salts of galacturonic acid.

引用

页码：423 / 435

页数：13

共 33 条

[1] THE METHANE FERMENTATION OF CITRUS-UNSHIU PEEL PRETREATED WITH FUNGUS ENZYMES [J].