LOCALIZATION OF ACID-PHOSPHATASE IN LACTATING RAT MAMMARY-GLANDS

被引：3

作者：

LEUNG, CT ^{[1
]}

MALEEFF, BE ^{[1
]}

WICKHAM, ED ^{[1
]}

FARRELL, HM ^{[1
]}

机构：

[1] USDA ARS,EASTERN REG RES CTR,600 E MERMAID LANE,PHILADELPHIA,PA 19118

来源：

PROTOPLASMA | 1990年 / 153卷 / 03期

关键词：

Acid phosphatase; Electron microscopy; Mammary glands; Subcellular fractions; Substrate specificity; Sulfhydryl agents; Tartrate;

D O I：

10.1007/BF01353999

中图分类号：

Q94 [植物学];

学科分类号：

071001 ;

摘要：

Localization of acid phosphatase in mammary glands of lactating rats was studied by both biochemical and cytochemical methods. Cytochemically, acid phosphatase activity was detected by using lead citrate as the capture agent for the inorganic phosphate released from p-nitrophenyl phosphate. The activity was predominantly localized in the lumina of the endomembrane system and in the milk that had been secreted into the alveolar lumen. Biochemically, acid phosphatase was present in all the subcellular fractions with higher activities in the membrane-associated fractions. The localization of tartrate-resistant acid phosphatases within the endomembrane system of fully lactating rat mammary tissue suggests a possible role for these enzymes in milk secretory processes. © 1990 Springer-Verlag.

引用

页码：149 / 156

页数：8

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