LITHIUM ENHANCES THE PROLIFERATION OF HL-60 PROMYELOCYTIC LEUKEMIA-CELLS

被引：22

作者：

BECKER, RW

TYOBEKA, EM

机构：

[1] Department of Biochemistry, University of the North, Sovenga, 0727

来源：

LEUKEMIA RESEARCH | 1990年 / 14卷 / 10期

关键词：

LITHIUM; HL-60; CELLS; PROLIFERATION; LEUKEMIA;

D O I：

10.1016/0145-2126(90)90177-B

中图分类号：

R73 [肿瘤学];

学科分类号：

100214 ;

摘要：

The effects of lithium, an agent used in the treatment of manic depression and to attenuate myelosuppression during chemotherapy, on HL-60 promyelocytic leukemia cells were investigated. By monitoring cell growth at varying concentrations (0-50 mM), as well as by following cell proliferation over 8 days, it was established that lithium stimulates HL-60 proliferation within a very narrow concentration range. Enhancement of growth was optimal at 5 mM, whereas concentrations above 10 mM were toxic. Time course studies revealed that the greatest increase in cell number occurred after 5-6 days in the presence of lithium. This was preceded by DNA synthesis reaching a maximum after 1-2 days. Viability of the cells decreased gradually after 3 days with 5 mM, but not with 2.5 mM. We suggest that HL-60 cells are a suitable model to further investigate possible mitogenic and cytotoxic effects of lithium in vitro.

引用

页码：879 / 884

页数：6

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