P-31 AND H-1-NMR PULSE SEQUENCES TO MEASURE LINESHAPES, T1Z AND T2E RELAXATION-TIMES IN BIOLOGICAL-MEMBRANES

被引：15

作者：

DUFOURC, EJ ^{[1
]}

MAYER, C ^{[1
]}

STOHRER, J ^{[1
]}

KOTHE, G ^{[1
]}

机构：

[1] UNIV STUTTGART,DEPT PHYS CHEM,W-7000 STUTTGART 80,GERMANY

来源：

JOURNAL DE CHIMIE PHYSIQUE ET DE PHYSICO-CHIMIE BIOLOGIQUE | 1992年 / 89卷 / 02期

关键词：

D O I：

10.1051/jcp/1992890243

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The dynamics of model membrane phospholipids has been monitored by phosphorus-31 solid state nuclear magnetic resonance (P-31 NMR). Radio frequency pulse sequences were designed to monitor spectral lineshapes, longitudinal (T1Z) and transverse (T2E) relaxation times dominated by P-31 chemical shielding anisotropy. Lineshapes and T2E are obtained by means of Hahn-echo sequences whereas T1Z is measured either by inversion or saturation methods coupled with the spin-echo sequence. The dipolar proton-phosphorus interaction was either suppressed by proton spin-lock or estimated through measurement of the maximum nuclear Overhauser enhancement (NOE).

引用

页码：243 / 252

页数：10

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