SUPRAMOLECULAR SELF-ASSEMBLY OF ESCHERICHIA-COLI GLUTAMINE-SYNTHETASE - EFFECTS OF PRESSURE AND ADENYLYLATION STATE ON DODECAMER STACKING

Escherichia coli glutamine synthetase is a dodecamer of identical subunits, consisting of two face-to-face hexameric rings. The enzymatic activity of GS is regulated by covalent attachment of an adenylyl group to each subunit, at the edge of the ring structure (Tyr-397). In the presence of Zn2+, Cu2+, Co2+, and other divalent metal ions, the free dodecamers self-organize into protein tabules [Miller et al. (1974) Arch, Biochem. Biophys. 163, 155-171]. Here, the temperature dependence and pressure dependence of the kinetics of Zn2+-induced self-assembly of GS tubules have been determined for the adenylylated and unadenylylated GS. The adenylylated enzyme exhibits a bimolecular rate constant for Zn2+-induced stacking that is 3-fold lower than for the unadenylylated GS at temperatures ranging from 0 to 25 degrees C. The enthalpy of activation, Delta H double dagger, far both adenylylated and unadenylylated GS increases from approximately 10 kcal/mol of dodecamer interface to 20 kcal/mol of dodecamer interface upon addition of 125 mM KCl to the reaction buffer. The Delta H double dagger values for adenylylated and unadenylylated GS are nearly identical, at each concentration of KCl, suggesting that entropic factors are responsible for the differences in rate of stacking for these forms of GS. Hydrostatic pressure markedly inhibits the stacking reaction for both adenylylated and unadenylylated GS. The activation volumes, Delta V double dagger(a), for stacking are increased from approximately 50 mL/mol of dodecamer interface in the absence of KCl to approximately 65 mL/mol of dodecamer interface in the presence of 125 mM KCl. The kinetics of pressure-induced disassembly of GS tubules were also examined. ''Pressure-jump'' experiments with preformed GS tubules indicate that rates of dissociation at atmospheric pressure, in the presence of 200 mu M Zn2+ and 125 mM KCl, are 1.1 x 10(-5) s(-1) and 1.9 x 10(-5) s(-1) for the unadenylylated and adenylylated GS, respectively. The Delta V double dagger(d) values for disassembly increase from approximately -63 mL/mol of dodecamer interface in the absence of KCl to approximately -55 mL/mol of dodecamer interface in the presence of 125 mM KCl. These results suggest that the encounter complex formed between dodecamers, en route to the stacked complex, is not extensively desolvated.; Furthermore, the experimentally determined rate constants for the forward and reverse reactions have been used to calculate apparent equilibrium dissociation constants. These Values range from 1.3 x 10(-10) M(-1) to 8.0 x 10(-9) M(-1), and they are increased by adenylylation and by addition of KCl.