RED-BLOOD-CELL SEPARATIONS BY GRAVITATIONAL FIELD-FLOW FRACTIONATION - INSTRUMENTATION AND APPLICATIONS

被引：28

作者：

BERNARD, A ^{[1
]}

PAULET, B ^{[1
]}

COLIN, V ^{[1
]}

CARDOT, PJP ^{[1
]}

机构：

[1] UNIV PARIS SUD, FAC PHARM, CHIM ANALYT & ELECTROCHIM ORGAN LAB, F-92296 CHATENAY MALABRY, FRANCE

来源：

TRAC-TRENDS IN ANALYTICAL CHEMISTRY | 1995年 / 14卷 / 06期

关键词：

D O I：

10.1016/0165-9936(95)91619-4

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

Field-flow fractionation (FFF), first described more than 20 years ago, is in constant development and, surprisingly, the simplest method, gravitational field-flow fractionation (GFFF), appears to be very useful in cell separations -although this is still at an early stage of development. We describe here the design of a very simple biocompatible separator which can directly replace the chromatographic column in an HPLC system. The relative complexity of the elution of cellular material is shown for the case of human red blood cells (RBC) of different characteristics and in this paper we show the versatility and selectivity of FFF methods in micron-scale separations.

引用

页码：266 / 273

页数：8

共 26 条

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