MUNICIPAL WASTE-WATER CONTAMINATION IN THE SOUTHERN-CALIFORNIA BIGHT .2. CYTOSOLIC DISTRIBUTION OF CONTAMINANTS AND BIOCHEMICAL EFFECTS IN FISH LIVERS

The objective of this study was to examine the cytosolic distribution of metals and oxygenated organic metabolites (MTBs), and biochemical effects, in livers of fish collected from both highly contaminated and less contaminated southern California coastal sites. Cytosolic extracts were separated by Sephadex G-75 column chromatography into high molecular weight ( greater than 20,000 daltons) enzyme-containing (ENZ) pools, medium molecular weight (3000-20,000 daltons) metallothionein- or metallothionein-like-containing (MT) pools, and low molecular weight ( less than 3000 daltons) glutathione-containing (GSH) pools. Additional study materials and methods, an study results are discussed in the paper.