ENHANCED SURVIVAL OF CULTURED DOPAMINE NEURONS BY TREATMENT WITH SOLUBLE EXTRACTS FROM CHEMICALLY DEAFFERENTIATED STRIATUM OF ADULT-RAT BRAIN

被引：42

作者：

NIIJIMA, K

ARAKI, M

OGAWA, M

NAGATSU, I

SATO, F

KIMURA, H

YOSHIDA, M

机构：

[1] JICHI MED SCH,DEPT ANAT,MINAMI KAWACHI,TOCHIGI 32904,JAPAN

[2] JICHI MED SCH,DEPT NEUROSURG,MINAMI KAWACHI,TOCHIGI 32904,JAPAN

[3] FUJITA HLTH UNIV,SCH MED,DEPT ANAT,TOYOAKE,AICHI,JAPAN

[4] SHIGA UNIV MED SCI,MOLEC NEUROBIOL RES CTR,OTSU,SHIGA 52021,JAPAN

来源：

BRAIN RESEARCH | 1990年 / 528卷 / 01期

关键词：

6-Hydroxydopamine; Cell culture; Dopamine neuron; Neurotrophic factor; Striatum;

D O I：

10.1016/0006-8993(90)90209-T

中图分类号：

Q189 [神经科学];

学科分类号：

071006 ;

摘要：

A soluble fraction was extracted from a chemically deafferentiated striatum of adult Wistar rats after unilateral lesioning of the nigrostriatal pathway by 6-hydroxydopamine (6-OHDA) injection. The soluble extract from the lesioned side enhanced the survival of cultured mesencephalic dopamine (DA) neurons of 14-day-old rat embryos as evidenced by quantitative counting of tyrosine hydroxylase-like immunoreactive cells. The neurotrophic activity of this striatal extract for DA neurons was highest 14 days after 6-OHDA injection and became negligible in 28 days. The extract showed no promoting effects on cultured γ-aminobutyric acid (GABA)-containing mesencephalic neurons. These observations indicate that the striatum of adult rats may initiate de novo synthesis of trophic substance(s) for DA neurons but not for GABA neurons when subjected to nigral dopaminergic deafferentiation. © 1990.

引用

页码：151 / 154

页数：4

共 27 条

[1] NEURAL CELL-ADHESION MOLECULE (N-CAM) ACCUMULATES IN DENERVATED AND PARALYZED SKELETAL-MUSCLES [J].