DETERMINATION OF HYDROXYPROLINE BY HIGH-PRESSURE LIQUID-CHROMATOGRAPHY

被引：28

作者：

GREEN, GD ^{[1
]}

REAGAN, K ^{[1
]}

机构：

[1] ORGANOGENESIS INC,CAMBRIDGE,MA 02142

来源：

ANALYTICAL BIOCHEMISTRY | 1992年 / 201卷 / 02期

关键词：

D O I：

10.1016/0003-2697(92)90337-7

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A rapid, precise, and simple HPLC method provides an assay of hydroxyproline from tissue extracts or solutions of collagen. Samples are hydrolyzed with 6 n HCl, derivatized with phenyl isothiocyanate, and chromatographed on a small, C18 reverse-phase HPLC column. Hydroxyproline (Hyp) is separated from other amino acids and detected by absorption at 254 nm. The method detects 0.40 to 36 μg of Hyp with a linear response. Separation requires a total of 6 min, including column cleanup and reequilibration. All components are commercially available, making this a convenient method for routine measurement of collagen concentration. © 1992.

引用

页码：265 / 269

页数：5

共 20 条

[1] DERIVATIZATION WITH 4-CHLORO-7-NITROBENZOFURAZAN FOR LIQUID-CHROMATOGRAPHIC DETERMINATION OF HYDROXYPROLINE IN COLLAGEN HYDROLYSATE
AHNOFF, M
GRUNDEVIK, I
ARFWIDSSON, A
FONSELIUS, J
PERSSON, BA
[J]. ANALYTICAL CHEMISTRY, 1981, 53 (03) : 485 - 489
[2] BERG RA, 1982, METHOD ENZYMOL, V82, P372
[3] APPLICATION OF HIGH-PRESSURE LIQUID-CHROMATOGRAPHY TO STUDIES OF COLLAGEN PRODUCTION BY ISOLATED CELLS IN CULTURE
CAMPA, JS
MCANULTY, RJ
LAURENT, GJ
[J]. ANALYTICAL BIOCHEMISTRY, 1990, 186 (02) : 257 - 263
[4] HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHIC DETERMINATION OF HYDROXYPROLINE AFTER DERIVATIZATION WITH 4-DIMETHYLAMINOAZOBENZENE-4'-SULPHONYL CHLORIDE
CASINI, A
MARTINI, F
NIERI, S
RAMARLI, D
FRANCONI, F
SURRENTI, C
[J]. JOURNAL OF CHROMATOGRAPHY, 1982, 249 (01): : 187 - 192
[5] COHEN SA, 1987, PROTEIN STRUCTURE FU, P207
[6] DAWSON CD, 1988, CLIN CHEM, V34, P1572
[7] DETERMINATION OF HYDROXYPROLINE IN TISSUE COLLAGEN HYDROLYSATE BY DERIVATIZATION AND ISOCRATIC REVERSED-PHASE HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY
DUNPHY, MJ
BHIDE, MV
SMITH, DJ
[J]. JOURNAL OF CHROMATOGRAPHY-BIOMEDICAL APPLICATIONS, 1987, 420 (02): : 394 - 397
[8] COLLAGEN - MOLECULAR DIVERSITY IN THE BODYS PROTEIN SCAFFOLD
EYRE, DR
[J]. SCIENCE, 1980, 207 (4437) : 1315 - 1322
[9] GREENSTEIN JP, 1961, CHEM AMINO ACIDS, V3, P2018
[10] HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHIC COLUMN SWITCHING METHOD FOR THE DETERMINATION OF HYDROXYPROLINE IN MEAT AND MEAT-PRODUCTS
JONES, AD
HOMAN, AC
FAVELL, DJ
HITCHCOCK, CHS
[J]. JOURNAL OF CHROMATOGRAPHY, 1986, 353 : 153 - 161

← 1 2 →