DETECTION AND CHARACTERIZATION OF A NOVEL BISEGMENTED DOUBLE-STRANDED-RNA VIRUS (PICOBIRNAVIRUS) FROM RABBIT FECES

被引:38
作者
GALLIMORE, C
LEWIS, D
BROWN, D
机构
[1] CENT PUBL HLTH LAB, ENTER & RESP DIS LAB, DIV VIRUS REFERENCE, 61 COLINDALE AVE, LONDON NW9 5HT, ENGLAND
[2] PUBL HLTH LAB, LEEDS, ENGLAND
关键词
D O I
10.1007/BF01309744
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
In two separate studies rabbits were fed orally with human and rabbit ''picobirnaviruses''. Polyacrylamide gel electrophoresis (PAGE) of nucleic acid extracted from faecal samples collected from inoculated rabbits revealed the presence of discrete equimolar bands, typical of picobirnaviruses, in several specimens. The genome profiles detected in both studies differed significantly from that of the inoculum suggesting that- passage of the inoculated picobirnaviruses had not taken place and that the bands were a co-incidental finding. The presence of rabbit picobirnaviruses was confirmed by characterization of the genome bands, as dsRNA by enzyme digestion and by their co-sedimentation in caesium chloride (CsCl) gradients with 32 nm virus particles at a buoyant density of 1.39 g/ml. Picobirnavirus genome segments varied in size in a range between 2.3-2.6 kilo base pairs (kbp) and 1.6-1.9 kbp for the slow and fast migrating bands, respectively. Immune electron microscopy of the picobirnavirus particles revealed round or slightly hexagonal particles with a smooth surface and a mean diameter of 30.7 nm. In one rabbit, an immune response, temporally associated with picobirnaviruses excretion, was demonstrated by immune electron microscopy (IEM) supporting the view that picobirnaviruses may be vertebrate viruses. Two antigenically distinct picobirnavirus strains were defined by IEM.
引用
收藏
页码:63 / 73
页数:11
相关论文
共 21 条
[1]  
ALFIERI A F, 1988, Arquivo Brasileiro de Medicina Veterinaria e Zootecnia, V40, P437
[2]   CRYPTIC PLANT-VIRUSES [J].
BOCCARDO, G ;
LISA, V ;
LUISONI, E ;
MILNE, RG .
ADVANCES IN VIRUS RESEARCH, 1987, 32 :171-214
[4]   EVALUATION OF STANDARD SEDIMENTATION COEFFICIENTS OF SODIUM RNA AND SODIUM DNA FROM SEDIMENTATION VELOCITY DATA IN CONCENTRATED NACL AND CSCL SOLUTIONS [J].
BRUNER, R ;
VINOGRAD, J .
BIOCHIMICA ET BIOPHYSICA ACTA, 1965, 108 (01) :18-&
[5]   6 GROUPS OF DOUBLE-STRANDED-RNA MYCOVIRUSES [J].
BUCK, KW ;
ACKERMANN, HW ;
BOZARTH, RF ;
BRUENN, JA ;
KOLTIN, Y ;
RAWLINSON, CJ ;
USHIYAMA, R ;
WOOD, HA .
INTERVIROLOGY, 1984, 22 (01) :17-23
[6]  
CHASEY D, 1990, VET REC, V126, P465
[7]   BIOPHYSICAL AND BIOCHEMICAL CHARACTERIZATION OF 5 ANIMAL VIRUSES WITH BISEGMENTED DOUBLE-STRANDED-RNA GENOMES [J].
DOBOS, P ;
HILL, BJ ;
HALLETT, R ;
KELLS, DTC ;
BECHT, H ;
TENINGES, D .
JOURNAL OF VIROLOGY, 1979, 32 (02) :593-605
[8]   THE MOLECULAR-BIOLOGY OF INFECTIOUS PANCREATIC NECROSIS VIRUS - A REVIEW [J].
DOBOS, P ;
ROBERTS, TE .
CANADIAN JOURNAL OF MICROBIOLOGY, 1983, 29 (04) :377-384
[9]   VIRUSES WITH BISEGMENTED DOUBLE-STRANDED-RNA IN PIG FECES [J].
GATTI, MSV ;
DECASTRO, AFP ;
FERRAZ, MMG ;
FIALHO, AM ;
PEREIRA, HG .
RESEARCH IN VETERINARY SCIENCE, 1989, 47 (03) :397-398
[10]  
HAMBLIN TJ, 1975, CLIN EXP IMMUNOL, V21, P101