RETROVIRAL TRANSFER OF THE N1SLACZ GENE INTO HUMAN CD34(+) CELL-POPULATIONS AND INTO TF-1 CELLS - FUTURE-PROSPECTS IN GENE-THERAPY

被引:29
作者
BAGNIS, C [1 ]
GRAVIS, G [1 ]
IMBERT, AM [1 ]
HERRERA, D [1 ]
ALLARIO, T [1 ]
GALINDO, R [1 ]
LOPEZ, M [1 ]
PAVON, C [1 ]
SEMPERE, C [1 ]
MANNONI, P [1 ]
机构
[1] INSERM,U119,F-13273 MARSEILLE,FRANCE
关键词
D O I
10.1089/hum.1994.5.11-1325
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
Few data are available concerning behavior of reimplanted human hematopoietic cells after autologous stem cell transplantation. This paper reports the possibility to transfer gene markers coding for beta-galactosidase (beta-Gal) activity by retroviral vectors into a human leukemic growth factor-dependent cell line, TF-1, and into human hematopoietic progenitors isolated from peripheral blood or bone marrow. Using various combinations of retroviral vectors and packaging cell lines, we demonstrated high expression of a bacterial beta-Gal activity induced by the LacZ gene, the nlsLacZ gene, or the Sh-ble/LacZ gene, in human hematopoietic cells. The expression of the nlsLacZ construct was stable until the end of the culture in infected CD34(+) cell-enriched cell populations, and a slow decrease of transgene expression was observed in a transduced TF-1 cell population during a 1-year long-term culture. Data obtained with the nlsLacZ gene demonstrate that both retroviral transfer and corresponding gene expression were not found to modify the pattern of cell proliferation and differentiation. These results open interesting prospectives for the use of the nlsLacZ gene to mark and follow the fate of progenitor cells isolated from patients with cancers prior to reimplantation.
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收藏
页码:1325 / 1333
页数:9
相关论文
共 37 条
[1]   HUMAN GENE-THERAPY [J].
ANDERSON, WF .
SCIENCE, 1992, 256 (5058) :808-813
[2]  
ANDREWS RG, 1986, BLOOD, V67, P842
[3]  
BAGNIS C, 1993, ONCOGENE, V8, P737
[4]   A SELECTABLE BIFUNCTIONAL BETA-GALACTOSIDASE--PHLEOMYCIN-RESISTANCE FUSION PROTEIN AS A POTENTIAL MARKER FOR EUKARYOTIC CELLS [J].
BARON, M ;
REYNES, JP ;
STASSI, D ;
TIRABY, G .
GENE, 1992, 114 (02) :239-243
[5]  
BENSINGER W, 1993, BLOOD, V81, P3158
[6]   ANTIGEN CD34+ MARROW-CELLS ENGRAFT LETHALLY IRRADIATED BABOONS [J].
BERENSON, RJ ;
ANDREWS, RG ;
BENSINGER, WI ;
KALAMASZ, D ;
KNITTER, G ;
BUCKNER, CD ;
BERNSTEIN, ID .
JOURNAL OF CLINICAL INVESTIGATION, 1988, 81 (03) :951-955
[7]   GENE MARKING TO DETERMINE WHETHER AUTOLOGOUS MARROW INFUSION RESTORES LONG-TERM HEMATOPOIESIS IN CANCER-PATIENTS [J].
BRENNER, MK ;
RILL, DR ;
HOLLADAY, MS ;
HESLOP, HE ;
MOEN, RC ;
BUSCHLE, M ;
KRANCE, RA ;
SANTANA, VM ;
ANDERSON, WF ;
IHLE, JN .
LANCET, 1993, 342 (8880) :1134-1137
[8]   GENE-MARKING TO TRACE ORIGIN OF RELAPSE AFTER AUTOLOGOUS BONE-MARROW TRANSPLANTATION [J].
BRENNER, MK ;
RILL, DR ;
MOEN, RC ;
KRANCE, RA ;
MIRRO, J ;
ANDERSON, WF ;
IHLE, JN .
LANCET, 1993, 341 (8837) :85-86
[9]   SAFE AND EFFICIENT GENERATION OF RECOMBINANT RETROVIRUSES WITH AMPHOTROPIC AND ECOTROPIC HOST RANGES [J].
DANOS, O ;
MULLIGAN, RC .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1988, 85 (17) :6460-6464
[10]   GENE-TRANSFER INTO NORMAL HUMAN HEMATOPOIETIC-CELLS USING INVITRO AND INVIVO ASSAYS [J].
DICK, JE ;
KAMELREID, S ;
MURDOCH, B ;
DOEDENS, M .
BLOOD, 1991, 78 (03) :624-634