STUDIES OF SPECIFIC GENE INDUCTION DURING APOPTOSIS OF CELL-LINES CONDITIONALLY IMMORTALIZED BY SV40

被引：11

作者：

GUENAL, I ^{[1
]}

MIGNOTTE, B ^{[1
]}

机构：

[1] UNIV VERSIALLES ST QUENTIN,F-78035 VERSAILLES,FRANCE

来源：

FEBS LETTERS | 1995年 / 374卷 / 03期

关键词：

SV40; P53; APOPTOSIS; IMMORTALIZATION; GENE EXPRESSION;

D O I：

10.1016/0014-5793(95)01157-A

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Inactivation of SV40 large T antigen in cells immortalized with conditional mutants leads to activation of p53 and apoptosis. We have analysed during this process the expression of genes induced by p53 or differentially expressed during apoptosis in other systems. We find an early induction of Waf1/Cip1. We also observe clusterin is induced during the process and displays a high level of expression in non-apoptotic cells, suggesting a protective role for clusterin. Other genes associated with thymocyte and lymphocyte apoptosis are not induced, showing that the pattern of gene induction is specific to the system studied.

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页码：384 / 386

页数：3

相关论文

共 32 条

[1] GENES NEWLY IDENTIFIED AS REGULATED BY GLUCOCORTICOIDS IN MURINE THYMOCYTES [J].

BAUGHMAN, G ;

HARRIGAN, MT ;

CAMPBELL, NF ;

NURRISH, SJ ;

BOURGEOIS, S .

MOLECULAR ENDOCRINOLOGY, 1991, 5 (05) :637-644

[2] ISOLATION AND CHARACTERIZATION OF TRANSCRIPTS INDUCED BY ANDROGEN WITHDRAWAL AND APOPTOTIC CELL-DEATH IN THE RAT VENTRAL PROSTATE [J].

BRIEHL, MM ;

MIESFELD, RL .

MOLECULAR ENDOCRINOLOGY, 1991, 5 (10) :1381-1388

[3] CELL-DEATH BY APOPTOSIS AND ITS PROTECTIVE ROLE AGAINST DISEASE [J].

BURSCH, W ;

OBERHAMMER, F ;

SCHULTEHERMANN, R .

TRENDS IN PHARMACOLOGICAL SCIENCES, 1992, 13 (06) :245-251

[4] INDUCTION OF THE TRPM-2 GENE IN CELLS UNDERGOING PROGRAMMED DEATH [J].

BUTTYAN, R ;

OLSSON, CA ;

PINTAR, J ;

CHANG, CS ;

BANDYK, M ;

NG, PY ;

SAWCZUK, IS .

MOLECULAR AND CELLULAR BIOLOGY, 1989, 9 (08) :3473-3481

[5] P53-DEPENDENT APOPTOSIS IN THE ABSENCE OF TRANSCRIPTIONAL ACTIVATION OF P53-TARGET GENES [J].

CAELLES, C ;

HELMBERG, A ;

KARIN, M .

NATURE, 1994, 370 (6486) :220-223

[6]

CHANG C, 1987, J BIOL CHEM, V262, P11901

[7] ISOLATION OF BIOLOGICALLY-ACTIVE RIBONUCLEIC-ACID FROM SOURCES ENRICHED IN RIBONUCLEASE [J].

CHIRGWIN, JM ;

PRZYBYLA, AE ;

MACDONALD, RJ ;

RUTTER, WJ .

BIOCHEMISTRY, 1979, 18 (24) :5294-5299

[8] REEVALUATION OF THE ROLE OF DE-NOVO PROTEIN-SYNTHESIS IN RAT THYMOCYTE APOPTOSIS [J].

CHOW, SC ;

PETERS, I ;

ORRENIUS, S .

EXPERIMENTAL CELL RESEARCH, 1995, 216 (01) :149-159

[9] BIOSYNTHESIS AND MOLECULAR-CLONING OF SULFATED GLYCOPROTEIN-2 SECRETED BY RAT SERTOLI CELLS [J].

COLLARD, MW ;

GRISWOLD, MD .

BIOCHEMISTRY, 1987, 26 (12) :3297-3303

[10] UBIQUITIN PATHWAY INVOLVEMENT IN HUMAN LYMPHOCYTE GAMMA-IRRADIATION-INDUCED APOPTOSIS [J].

DELIC, J ;

MORANGE, M ;

MAGDELENAT, H .

MOLECULAR AND CELLULAR BIOLOGY, 1993, 13 (08) :4875-4883

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