MOLECULAR ANALYSIS OF OPAQUE-2 ALLELES FROM ZEA-MAYS L REVEALS THE NATURE OF MUTATIONAL EVENTS AND THE PRESENCE OF A HYPERVARIABLE REGION IN THE 5' PART OF THE GENE

Ten recessive Opaque-2 (O2) alleles of independent origin were characterized at the molecular level. The results revealed a high level of polymorphism at the O2 locus. In addition, our data suggest the possible cause for the recessive character of some of the alleles investigated, and allow us to infer some conclusions concerning the degree of relationship between the o2 mutations. Comparison of genomic sequences spanning the first exon and obtained from a series of wild-type and recessive alleles revealed the presence of a hypervariable region, involving different dipeptides, in the N-terminal part of the O2 protein.