SEPARATION OF L-VALINE FROM FERMENTATION BROTHS USING A SUPPORTED LIQUID MEMBRANE

被引:46
作者
DEBLAY, P [1 ]
MINIER, M [1 ]
RENON, H [1 ]
机构
[1] ECOLE NATL SUPER MINES,CTR REACTEURS & PROC,F-75006 PARIS,FRANCE
关键词
D O I
10.1002/bit.260350203
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
A carrier‐mediated counter transport process is proposed to separate and to purify an amino acid produced by microbial fermentation. The case of L‐valine permeation through a liquid membrane, constituted by a solution of Aliquat 336 in decanol and supported by a hydrophobic microporous membrane, is reported. A mathematical model was developed to estimate distribution coefficients and permeabilities and to predict the influence of hydrodynamic and pH conditions on supported liquid membrane (SLM) performances. Optimum conditions for the transport and the concentration of valine were achieved with synthetic aqueous valine solutions. Series of experiments on fermentation broths, where molasses and biomass contents were varied, permitted pointing out the role of the broth composition on the kinetics and yields of separation. The selectivity of transport of valine by an Aliquat 336/decanol liquid membrane was about 10 toward molasses dyes, 100 toward glucose, and beyond 1000 toward sucrose. This allowed us to achieve the recovery and one step of purification of the product in a single operation. The stability of the Aliquat 336/decanol liquid membrane was sufficient to ensure a selective transport of valine during a continuous run lasting 18 days. Copyright © 1990 John Wiley & Sons, Inc.
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页码:123 / 131
页数:9
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