TRANSIENT EXPRESSION OF A P58 PROTEIN-KINASE CDNA ENHANCES MAMMALIAN GLYCOSYLTRANSFERASE ACTIVITY

被引：39

作者：

BUNNELL, BA ^{[1
]}

ADAMS, DE ^{[1
]}

KIDD, VJ ^{[1
]}

机构：

[1] UNIV ALABAMA,DEPT CELL BIOL,BIRMINGHAM,AL 35294

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 1990年 / 171卷 / 01期

关键词：

D O I：

10.1016/0006-291X(90)91376-4

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The effect of expression of a p58 protein kinase on mammalian β-1,4 galactosyltransferase enzyme activity was examined in vitro and in vivo. We found that p58 protein kinase expression enhanced galactosyltransferase enzyme activity approximately three-fold in vivo when compared to reporter gene activity. Galactosyltransferase enzyme activity was also substantially reduced in vitro when dephosphorylated, or when p58 specific antibodies were used to inhibit kinase activity. These results suggest that galactosyltransferase activity is influenced by phosphorylation, and that the p58 protein kinase may mediate this effect. © 1990.

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页码：196 / 203

页数：8

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