MITOCHONDRIAL MALATE-DEHYDROGENASE FROM CORN - PURIFICATION OF MULTIPLE FORMS

被引:46
作者
HAYES, MK
LUETHY, MH
ELTHON, TE
机构
[1] UNIV NEBRASKA,SCH BIOL SCI,LINCOLN,NE 68588
[2] UNIV NEBRASKA,CTR BIOTECHNOL,LINCOLN,NE 68588
关键词
D O I
10.1104/pp.97.4.1381
中图分类号
Q94 [植物学];
学科分类号
071001 ;
摘要
A method to fractionate com (Zea mays L B73) mitochondria into soluble proteins, high molecular weight soluble proteins, and membrane proteins was developed. These fractions were analyzed by both sodium dodecyl sulfate-polyacrylamide gel electrophoresis and assays of mitochondrial enzyme activities. The Krebs cycle were enriched in the soluble fraction Malate dehydrogenase has been purified from the soluble fraction by a two-step fast protein liquid chromatography method. Six different malate dehydrogenase peaks were obtained from the Mono Q column. These peaks were individually purified using a Phenyl Superose column. Sodium dodecyl sulfate-polyacrylamide gel electrophoresis of the purified peaks showed that three of the isoenzymes consisted of different homodimers (I, III, VI) and three were different heterodimers (II, IV, V). Apparent molecular masses of the three different monomer subunits were 37, 38, and 39 kilodaltons. Nondenaturing gel analysis of the malate dehydrogenase peaks showed that each Mono Q peak contained a band of malate dehydrogena activity with different mobility. These observations are consistent with three nuclear genes encoding corn mitochondrial malate dehydrogenase. Polyclonal antibodies raised against purified malate dehydrogenase were used to identify the gene products using Western blots of two-dimensional gels.
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页码:1381 / 1387
页数:7
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