A 17.6 KBP REGION LOCATED UPSTREAM OF THE RABBIT WAP GENE DIRECTS HIGH-LEVEL EXPRESSION OF A FUNCTIONAL HUMAN PROTEIN VARIANT IN TRANSGENIC MOUSE MILK

被引：36

作者：

BISCHOFF, R

DEGRYSE, E

PERRAUD, F

DALEMANS, W

ALHADJI, D

THEPOT, D

DEVINOY, E

HOUDEBINE, LM

PAVIRANI, A

机构：

[1] TRANSGENE SA,11 RUE MOLSHEIM,F-67082 STRASBOURG,FRANCE

[2] INRA,UNITE DIFFERENCIAT CELLULAIRE,F-78350 JOUY EN JOSAS,FRANCE

来源：

FEBS LETTERS | 1992年 / 305卷 / 03期

关键词：

RABBIT; WAP GENE; ALPHA-1-ANTITRYPSIN; TRANSGENIC MOUSE; MILK; PLASMA KALLIKREIN;

D O I：

10.1016/0014-5793(92)80683-8

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

We have investigated whether DNA regions present in the rabbit whey acidic protein (WAP) promoter/5' flanking sequence could potentially confer, in vivo, high level expression of reporter genes. Transgenic mice were generated expressing a variant of human alpha-1-antitrypsin, which has inhibitory activity against plasma kallikrein under the control of a 17.6 kbp DNA fragment located upstream of the rabbit WAP gene. Up to 10 mg/ml of active and correctly processed recombinant protein were detected in mouse milk, thus suggesting that the far upstream DNA sequences from the rabbit WAP gene might be useful for engineering efficient protein production in the mammary glands of transgenic animals.

引用

页码：265 / 268

页数：4

共 24 条

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[Anonymous], 1986, MANIPULATING MOUSE E

[2] HIGH-LEVEL EXPRESSION OF BIOLOGICALLY-ACTIVE HUMAN ALPHA-1-ANTITRYPSIN IN THE MILK OF TRANSGENIC MICE [J].