COMPARISON OF THE X-RAY STRUCTURE OF NATIVE RUBREDOXIN FROM PYROCOCCUS-FURIOSUS WITH THE NMR STRUCTURE OF THE ZINC-SUBSTITUTED PROTEIN

被引：41

作者：

BLAKE, PR

DAY, MW

HSU, BT

JOSHUATOR, L

PARK, JB

HARE, DR

ADAMS, MWW

REES, DC

SUMMERS, MF

机构：

[1] UNIV MARYLAND,DEPT CHEM & BIOCHEM,CATONSVILLE,MD 21228

[2] CALTECH,DIV CHEM & CHEM ENGN,PASADENA,CA 91125

[3] HARE RES INC,BOTHELL,WA 98011

[4] UNIV GEORGIA,DEPT BIOCHEM,ATHENS,GA 30602

[5] UNIV GEORGIA,CTR MET ENZYME STUDIES,ATHENS,GA 30602

来源：

PROTEIN SCIENCE | 1992年 / 1卷 / 11期

关键词：

DISTANCE GEOMETRY; HYPERTHERMOPHILE; IRON SULFUR PROTEIN; NMR STRUCTURE; PROTEIN STRUCTURE; RUBREDOXIN; X-RAY STRUCTURE; ZINC SUBSTITUTION;

D O I：

10.1002/pro.5560011113

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The three-dimensional X-ray structures of the oxidized and reduced forms of rubredoxin from Pyrococcus furiosus, determined at - 161-degrees-C, and the NMR structure of the zinc-substituted protein, determined in solution at 45-degrees-C, are compared. The NMR and X-ray structures, which were determined independently, are very similar and lead to similar conclusions regarding the interactions that confer hyperthermostability.

引用

页码：1522 / 1525

页数：4

共 2 条

[1] SOLUTION-STATE STRUCTURE BY NMR OF ZINC-SUBSTITUTED RUBREDOXIN FROM THE MARINE HYPERTHERMOPHILIC ARCHAEBACTERIUM PYROCOCCUS-FURIOSUS
BLAKE, PR
PARK, JB
ZHOU, ZH
HARE, DR
ADAMS, MWW
SUMMERS, MF
[J]. PROTEIN SCIENCE, 1992, 1 (11) : 1508 - 1521
[2] X-RAY CRYSTAL-STRUCTURES OF THE OXIDIZED AND REDUCED FORMS OF THE RUBREDOXIN FROM THE MARINE HYPERTHERMOPHILIC ARCHAEBACTERIUM PYROCOCCUS-FURIOSUS
DAY, MW
HSU, BT
JOSHUATOR, L
PARK, JB
ZHOU, ZH
ADAMS, MWW
REES, DC
[J]. PROTEIN SCIENCE, 1992, 1 (11) : 1494 - 1507

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