CLONING AND CHARACTERIZATION OF FULL-LENGTH CDNAS CODING FOR THE DNA TOPOISOMERASE-II-BETA FROM CHINESE-HAMSTER LUNG-CELLS SENSITIVE AND RESISTANT TO 9-OH-ELLIPTICINE

被引：22

作者：

DEREUDDRE, S ^{[1
]}

FREY, S ^{[1
]}

DELAPORTE, C ^{[1
]}

JACQUEMINSABLON, A ^{[1
]}

机构：

[1] INST GUSTAVE ROUSSY,UNIV BIOCHIM ENZYMOL,CNRS,URA 147,F-94805 VILLEJUIF,FRANCE

来源：

BIOCHIMICA ET BIOPHYSICA ACTA-GENE STRUCTURE AND EXPRESSION | 1995年 / 1264卷 / 02期

关键词：

CDNA; TOPOISOMERASE II BETA; 9-OH-ELLIPTICINE; (CHINESE HAMSTER LUNG CELL);

D O I：

10.1016/0167-4781(95)00164-C

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

DNA topoisomerase II beta cDNAs from Chinese hamster lung cells sensitive (DC-3F) and resistant to 9-OH-ellipticine (DC-3F/90-HE) were isolated. In the sensitive cells, the sequence defines an open reading frame of 4839 nucleotides, and extends over a 323 nucleotides untranslated region up to the putative polyadenylation site. The deduced amino acid sequence predicts a protein with 1612 amino acids in length and a calculated molecular mass of approx. 182 kDa. The cDNAs from the resistant cells only differs by one mutation at position 1710 which converts a Trp codon (TGG) to a stop codon (TGA). This mutation accounts for the loss of DNA topoisomerase II beta in the 9-OH-ellipticine resistant cells.

引用

页码：178 / 182

页数：5

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