EVALUATION OF AN AUTOMATIC HPLC ANALYZER FOR THALASSEMIA AND HEMOGLOBIN-VARIANTS SCREENING

被引:20
作者
GALANELLO, R
BARELLA, S
GASPERINI, D
PERSEU, L
PAGLIETTI, E
SOLLAINO, C
PADERI, L
PIRRONI, MG
MACCIONI, L
MOSCA, A
机构
[1] Istituto di Clinica e Biologia deWEtà Evolutiva, Università degli Studi di Cagliari, 09100 Cagliari, Via Jenner s/n
来源
JOURNAL OF AUTOMATIC CHEMISTRY | 1995年 / 17卷 / 02期
关键词
D O I
10.1155/S1463924695000125
中图分类号
Q5 [生物化学];
学科分类号
071010 ; 081704 ;
摘要
In this paper the authors report the evolution of a new automatic HPLC analyser for screening haemoglobinopathies. HbA(2) and F determinations are accurate and reproducible. The analysis time is short (6.5 min) and there is a good separation between the HbA(2) values of beta-thalassemia carriers from normals and alpha-thalassemia carriers, with no overlap between these groups. In addition, the system is also able to detect and quantitate most of the haemoglobin variants, particularly those (HbS, HbC, HbE and Hb Lepore) able to interact with beta-thalassemia and could make haemoglobin electrophoresis unnecessary in all samples. The ease of operation and the limited technical work make this system especially suitable for laboratories with a high workload and allow the cost of screening to be reduced.
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页码:73 / 76
页数:4
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