INFLUENCE OF AIRBORNE SUSPENDED MATTER ON MITOTIC CELL-DIVISION

被引：15

作者：

MOTYKIEWICZ, G ^{[1
]}

HADNAGY, W ^{[1
]}

SEEMAYER, NH ^{[1
]}

SZELIGA, J ^{[1
]}

TKOCZ, A ^{[1
]}

CHORAZY, M ^{[1
]}

机构：

[1] UNIV DUSSELDORF,MED INST ENVIRONM HYG,W-4000 DUSSELDORF 1,GERMANY

来源：

MUTATION RESEARCH | 1991年 / 260卷 / 02期

关键词：

AIR POLLUTANTS; MITOTIC ARREST; MAMMALIAN CELL CULTURE;

D O I：

10.1016/0165-1218(91)90008-A

中图分类号：

Q3 [遗传学];

学科分类号：

071007 ; 090102 ;

摘要：

The effect of organic extracts of airborne suspended matter collected in the highly polluted industrial region of Silesia (Poland) on mitotic cell division was evaluated in the Chinese hamster V79 cell line. Crude benzene extracts as well as sequential elution solvent chromatography (SESC) fractions were investigated for their ability to affect the mitotic index, the proportion of anaphases-telophases to metaphases (AT/M ratio), the cloning efficiency and to produce aneuploid cells. The incidence of cell division disturbances in V79 cells exposed to extracts increased in a concentration-dependent manner. Mitotic arrest, manifested as a highly increased mitotic index and a concomitant decrease in the AT/M ratio, was found for the crude extract at a dose corresponding to 0.75 m3 of air. Comparable effects were noticed for SESC fraction 4, probably containing monophenol compounds. A strong dose-dependent reduction of cloning efficiency of V79 cells demonstrated cytotoxic activity of both the crude extract and fraction 4.

引用

页码：195 / 202

页数：8

共 25 条

[1] CHARACTERIZATION OF MICROBIAL MUTAGENS IN COMPLEX SAMPLES - METHODOLOGY AND APPLICATION [J].

ALFHEIM, I ;

BJORSETH, A ;

MOLLER, M .

CRC CRITICAL REVIEWS IN ENVIRONMENTAL CONTROL, 1984, 14 (02) :91-150

[2] THE PROSPECTS FOR A SIMPLIFIED AND INTERNATIONALLY HARMONIZED APPROACH TO THE DETECTION OF POSSIBLE HUMAN CARCINOGENS AND MUTAGENS [J].

ASHBY, J .

MUTAGENESIS, 1986, 1 (01) :3-16

[3] 1,6-DINITROPYRENE CAUSES SPINDLE DISTURBANCES AND CHROMOSOMAL DAMAGE IN V79 CHINESE-HAMSTER CELLS [J].

BAUCHINGER, M ;

SCHMID, E ;

WIEBEL, FJ ;

ROSCHER, E .

MUTATION RESEARCH, 1988, 208 (3-4) :213-218

[4] FRACTIONATION AND STRUCTURAL CHARACTERIZATION OF COAL LIQUIDS [J].

FARCASIU, M .

FUEL, 1977, 56 (01) :9-14

[5] CHEMICALLY-INDUCED ANEUPLOIDY IN MAMMALIAN-CELLS IN CULTURE [J].

GALLOWAY, SM ;

IVETT, JL .

MUTATION RESEARCH, 1986, 167 (1-2) :89-105

[6] INDUCTION OF C-TYPE METAPHASES AND ANEUPLOIDY IN CULTURES OF V79 CELLS EXPOSED TO EXTRACT OF AUTOMOBILE EXHAUST PARTICULATES [J].

HADNAGY, W ;

SEEMAYER, NH .

MUTAGENESIS, 1986, 1 (06) :445-448

[7] COMPARATIVE-STUDY OF SISTER-CHROMATID EXCHANGES AND CHROMOSOMAL-ABERRATIONS INDUCED BY AIRBORNE-PARTICULATES FROM AN URBAN AND A HIGHLY INDUSTRIALIZED LOCATION IN HUMAN-LYMPHOCYTE CULTURES [J].

HADNAGY, W ;

SEEMAYER, NH ;

TOMINGAS, R ;

IVANFY, K .

MUTATION RESEARCH, 1989, 225 (1-2) :27-32

[8] CYTOGENETIC EFFECTS OF AIRBORNE PARTICULATE MATTER IN HUMAN-LYMPHOCYTES INVITRO [J].

HADNAGY, W ;

SEEMAYER, NH ;

TOMINGAS, R .

MUTATION RESEARCH, 1986, 175 (02) :97-101

[9] CYTO-TOXIC AND GENOTOXIC EFFECTS OF EXTRACT OF PARTICULATE EMISSION FROM A GASOLINE-POWERED ENGINE [J].

HADNAGY, W ;

SEEMAYER, NH .

ENVIRONMENTAL AND MOLECULAR MUTAGENESIS, 1988, 12 (04) :385-396

[10]

HSU TC, 1986, CHEM MUTAGENS, V10, P155

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