IMMUNOASSAY METHOD FOR THE DETERMINATION OF IMMUNOGLOBULIN-G USING BACTERIAL MAGNETIC PARTICLES

被引：105

作者：

NAKAMURA, N ^{[1
]}

HASHIMOTO, K ^{[1
]}

MATSUNAGA, T ^{[1
]}

机构：

[1] TOKYO UNIV AGR & TECHNOL, DEPT BIOTECHNOL, KOGANEI, TOKYO 184, JAPAN

来源：

ANALYTICAL CHEMISTRY | 1991年 / 63卷 / 03期

关键词：

D O I：

10.1021/ac00003a015

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

We have developed a novel immunoassay method using bacterial magnetic particles for the determination of immunoglobulin G (IgG). Fluorescein isothiocyanate (FITC) conjugated anti IgG-bacterial magnetic particles were prepared. The fluorescence quenching caused by agglutination of FITC-anti IgG antibody-bacterial magnetic particle conjugates was measured by using a fluorescence spectrophotometer. The aggregates based on specific immunoreaction were separated by a gelatin solution. The aggregation of bacterial magnetic particle conjugates was enhanced by application of a magnetic field. The relative fluorescence intensity correlated linearly with a concentration of IgG in the range 0.5-100 ng/mL.

引用

页码：268 / 272

页数：5

共 25 条

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