THE SYNTHESIS AND PROPERTIES OF OLIGODEOXYRIBONUCLEOTIDES CONTAINING N6-METHOXYADENINE

被引：36

作者：

NISHIO, H ^{[1
]}

ONO, A ^{[1
]}

MATSUDA, A ^{[1
]}

UEDA, T ^{[1
]}

机构：

[1] HOKKAIDO UNIV,FAC PHARMACEUT SCI,KITA-12,NISHI-6,KITA KU,SAPPORO,HOKKAIDO 060,JAPAN

来源：

NUCLEIC ACIDS RESEARCH | 1992年 / 20卷 / 04期

关键词：

D O I：

10.1093/nar/20.4.777

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Oligodeoxyribonucleotides containing N6-methoxyadenine (M) have been synthesized. The order of stability of duplexes consisting of synthesized oligodeoxyribonucleotides, 5'd(CCTGGTAXCAGGTCC)3'-5'd(GGACCTGNTACCAGG)3' (X = M, A, G. N = A, G, T, C), was M: A (Tm = 52-degrees-C) > M: T (50-degrees-C) > M: G (48-degrees-C) > M: C (46-degrees-C) observed by thermal denaturation in a buffer of 0.01 M Na cacodylate, and 0.1 M NaCl at pH 7.0. The Tms are within a range of 6 degrees of difference, which is smaller than those of Tms of the duplexes containing A:N pairs (11 degrees) and G:N pairs (11 degrees). DNA replication study on a template-primer system, 5'd(P-32-CAGCTTTCGC)3' 3'd(GTCGAAAGCGMAGTCG)5', showed that TTP and dCTP were incorporated into DNA strands at a site opposite to M by Klenow DNA polymerase, but dATP and dGTP were not.

引用

页码：777 / 782

页数：6

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