BRAIN-DERIVED PEPTIDES REGULATE THE STEADY-STATE LEVELS AND INCREASE STABILITY OF THE BLOOD-BRAIN-BARRIER GLUT1 GLUCOSE-TRANSPORTER MESSENGER-RNA

被引：33

作者：

BOADO, RJ ^{[1
]}

机构：

[1] UNIV CALIF LOS ANGELES,SCH MED,BRAIN RES INST,LOS ANGELES,CA 90024

来源：

NEUROSCIENCE LETTERS | 1995年 / 197卷 / 03期

关键词：

BLOOD-BRAIN BARRIER; GLUCOSE TRANSPORTER; GLUT1; CEREBROLYSIN; GENE REGULATION; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION;

D O I：

10.1016/0304-3940(95)11930-U

中图分类号：

Q189 [神经科学];

学科分类号：

071006 ;

摘要：

The blood-brain barrier (BBB) GLUT1 glucose transporter gene expression is known to be regulated by putative brain trophic factors. Therefore, the present study investigated the effect of a brain-derived peptide rich preparation containing a neurotrophic factor-like action [Cerebrolysin (Cl), EBEWE, Austria]. In cultures of brain capillary endothelial cells, Cl induced a transient increase in the abundance of BBB-GLUT1 relative to actin measured by reverse transcription-polymerase chain reaction during the first 2 h of incubation, whereas a significant reduction in the GLUT1 transcript was observed at 20 and 48 h. In addition, Cl abolished the fall in GLUT1 levels induced by actinomycin D. The present data suggest that brain-derived factors in Cl are able to modulate the expression of the BBB-GLUT1 gene increasing the BBB-GLUT1 transcript stability.

引用

页码：179 / 182

页数：4

共 26 条

[1]

AGGARWAL BB, 1984, J BIOL CHEM, V259, P686

[2]

AKAI F, 1992, HISTOL HISTOPATHOL, V7, P213

[3] ASTROCYTE-MEDIATED INDUCTION OF TIGHT JUNCTIONS IN BRAIN CAPILLARY ENDOTHELIUM - AN EFFICIENT INVITRO MODEL [J].

ARTHUR, FE ;

SHIVERS, RR ;

BOWMAN, PD .

DEVELOPMENTAL BRAIN RESEARCH, 1987, 36 (01) :155-159

[4]

BOADO R J, 1990, Molecular and Cellular Neuroscience, V1, P224, DOI 10.1016/1044-7431(90)90005-O

[5] THE BRAIN-TYPE GLUCOSE TRANSPORTER MESSENGER-RNA IS SPECIFICALLY EXPRESSED AT THE BLOOD-BRAIN-BARRIER [J].

BOADO, RJ ;

PARDRIDGE, WM .

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 1990, 166 (01) :174-179

[6] A ONE-STEP PROCEDURE FOR ISOLATION OF POLY(A)+ MESSENGER-RNA FROM ISOLATED BRAIN CAPILLARIES AND ENDOTHELIAL-CELLS IN CULTURE [J].

BOADO, RJ ;

PARDRIDGE, WM .

JOURNAL OF NEUROCHEMISTRY, 1991, 57 (06) :2136-2139

[7]

BOADO RJ, 1994, J NEUROCHEM, V62, P2085

[8] ENHANCED EXPRESSION OF THE BLOOD-BRAIN-BARRIER GLUT1 GLUCOSE-TRANSPORTER GENE BY BRAIN-DERIVED FACTORS [J].

BOADO, RJ ;

WANG, L ;

PARDRIDGE, WM .

MOLECULAR BRAIN RESEARCH, 1994, 22 (1-4) :259-267

[9] GENE-EXPRESSION OF GLUT3 AND GLUT1 GLUCOSE TRANSPORTERS IN HUMAN BRAIN-TUMORS [J].

BOADO, RJ ;

BLACK, KL ;

PARDRIDGE, WM .

MOLECULAR BRAIN RESEARCH, 1994, 27 (01) :51-57

[10] GLUCOSE DEPRIVATION CAUSES POSTTRANSCRIPTIONAL ENHANCEMENT OF BRAIN CAPILLARY ENDOTHELIAL GLUCOSE TRANSPORTER GENE-EXPRESSION VIA GLUT1 MESSENGER-RNA STABILIZATION [J].

BOADO, RJ ;

PARDRIDGE, WM .

JOURNAL OF NEUROCHEMISTRY, 1993, 60 (06) :2290-2296

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