[H-3] DIHYDROTETRABENAZINE, A NEW MARKER FOR THE VISUALIZATION OF DOPAMINERGIC DENERVATION IN THE RAT STRIATUM

被引：47

作者：

MASUO, Y ^{[1
]}

PELAPRAT, D ^{[1
]}

SCHERMAN, D ^{[1
]}

ROSTENE, W ^{[1
]}

机构：

[1] INST BIOL PHYSICOCHIM,PARIS,FRANCE

来源：

NEUROSCIENCE LETTERS | 1990年 / 114卷 / 01期

关键词：

6-Hydroxydopamine; Autoradiography; Dihydrotetrabenazine; Dopamine; Nigral lesion; Striatum; Tyrosine hydroxylase;

D O I：

10.1016/0304-3940(90)90426-A

中图分类号：

Q189 [神经科学];

学科分类号：

071006 ;

摘要：

[3H]Dihydrotetrabenzine ([3H]TBZOH), a derivative of the monoamine depleting agent, tetrabenazine, has been shown to bind to the vesicular monoamine transporter. We studied the effect of unilateral nigral lesion with 6-hydroxydopamine on [3H]TBZOH binding in the striatum by means of quantitative autoradiography. Topographical analysis of striatal [3H]TBZOH binding showed in control rats a pattern similar to the known distribution of dopaminergic innervation, and allowed to visualize dopaminergic denervation in lesioned rats. A good correlation was found between striatal [3H]TBZOH binding levels and tyrosine hydroxylase activities in similar areas on adjacent slices following 6-OHDA lesions with varying severity. These data reveal that autoradiography of [3H]TBZOH binding is a good index for the visualization and quantitation of dopaminergic nerve terminals integrity in the striatum. © 1990.

引用

页码：45 / 50

页数：6

共 15 条

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