EFFECT OF INVIVO CONTACT BETWEEN BLOOD AND DIALYSIS MEMBRANES ON PROTEIN CATABOLISM IN HUMANS

被引:185
作者
GUTIERREZ, A
ALVESTRAND, A
WAHREN, J
BERGSTROM, J
机构
[1] KAROLINSKA INST,HUDDINGE UNIV HOSP,DEPT RENAL MED,K56,S-14186 HUDDINGE,SWEDEN
[2] KAROLINSKA INST,HUDDINGE UNIV HOSP,DEPT CLIN PHYSIOL,S-14186 HUDDINGE,SWEDEN
[3] KAROLINSKA INST,HUDDINGE UNIV HOSP,CTR CLIN RES,S-14186 HUDDINGE,SWEDEN
关键词
D O I
10.1038/ki.1990.230
中图分类号
R5 [内科学]; R69 [泌尿科学(泌尿生殖系疾病)];
学科分类号
1002 ; 100201 ;
摘要
To investigate whether the contact between blood and dialysis membranes might induce muscle protein degradation, the exchange of free amino acids across leg tissues was measured by catheterization technique in three groups of healthy subjects before and after a 150 minute sham-hemodialysis procedure (SHDP), that is in vivo passage of blood (100 ml/min) through a dialyzer but with no circulating dialysate. Dialyzers with either regenerated cellulose membrane (group CU, N = 10 and group CU-IND, N = 6) or polyacrylonitrile membrane (group AN, N = 8) were used In group CU-IND indomethacin was administered before (100 mg) and at the end (50 mg) of SHDP. Leg blood flow was measured by venous occlusion plethysmography. In group CU net leg release of tyrosine and phenylalanine increased from 3.4 ± 0.8 and 3.6 ± 0.8 nmol/min/100 g tissue, respectively, before SHDP to 7.8 ± 1.8 and 8.3 ± 1.8 nmol/min/ 100 g tissue, respectively, at 345 minutes after the start of SHDP (P < 0.01). The total release of all measured amino acids increased from 148 ± 31 to 309 ± 50 nmol/min/100 g tissue (P < 0.01). The results indicate that interaction between blood and regenerated cellulose membranes leads to accelerated net protein breakdown. In group CU-IND no change in leg amino acid release was observed following SHDP, suggesting that the increased net protein catabolism is mediated by prostaglandins. Sham hemodialysis using AN membranes did not result in increased amino acid efflux from leg tissues, implying that the protein catabolic effect of blood-membrane contact depends on the biochemical properties of dialyser.
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页码:487 / 494
页数:8
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