DEMONSTRATION OF SEROLOGICAL RELATIONSHIPS AMONG ISOLATES OF BARLEY YELLOW DWARF VIRUS BY USING POLYCLONAL AND MONOCLONAL-ANTIBODIES

被引:43
作者
DIACO, R
LISTER, RM
HILL, JH
DURAND, DP
机构
[1] IOWA STATE UNIV SCI & TECHNOL, DEPT PLANT PATHOL SEED & WEED SCI, AMES, IA 50011 USA
[2] IOWA STATE UNIV SCI & TECHNOL, DEPT MICROBIOL, AMES, IA 50011 USA
[3] PURDUE UNIV, DEPT BOT & PLANT PATHOL, W LAFAYETTE, IN 47907 USA
关键词
D O I
10.1099/0022-1317-67-2-353
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
Hybridomas secreting monoclonal antibodies against three isolates of barley yellow dwarf virus (BYDV) were established. Two monoclonal antibody preparations were generated against the MAV isolate, one against RPV and six against P-PAV. None of the monoclonal antibody preparations reacted with healthy host components. Reactions of monoclonal antibodies, or unlabelled polyclonal antisera, in an indirect enzyme-linked immunosorbent assay (ELISA) indicated that all three virus isolates share a common epitope. BYDV particles dissociated when incubated in carbonate-bicarbonate coating buffer at pH 9.6 but could be stabilized by prior dialysis against 2% formaldehyde or 2% glutaraldehyde. In indirect ELISA, unlabelled polyclonal antisera bound to both stabilized and dissociated particles of homologous and heterologous BYDV isolates. However, conjugated polyclonal antisera were incapable of binding to dissociated particles or to stabilized particles of heterologous isolates. Experiments with monoclonal antibodies in a competition ELISA indicated the presence of at least two epitopes on the coat protein of P-PAV.
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页码:353 / 362
页数:10
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