CHANGES IN CYTOKININS BEFORE AND DURING EARLY FLOWER BUD DIFFERENTIATION IN LYCHEE (LITCHI-CHINENSIS SONN)

Lychee (Litchi chinensis) has been analyzed for cytokinins in buds before and after flower bud differentiation, using reverse-phase high performance liquid chromatography in combination with Amaranthus bioassay and gas chromatography-mass spectrometry-selected ion monitoring. Four cytokinins, zeatin, zeatin riboside, N6-(delta-2-isopentenyl)adenine, and N6-(delta-6-isopentenyl) adenine riboside, were detected in buds. There was an increase of cytokinin activity in the buds during flower bud differentiation. In dormant buds, the endogenous cytokinin content was low, and the buds did not respond to exogenous cytokinin application. Application of kinetin promotes flower bud differentiation significantly after bud dormancy. These results are interpreted as an indication that the increase in endogeneous cytokinin levels during flower bud differentiation may be correlative rather than the cause of flower bud initiation.