OOCYTE DYSMORPHISM AND ANEUPLOIDY IN MEIOTICALLY MATURE HUMAN OOCYTES AFTER OVARIAN STIMULATION

被引：150

作者：

VANBLERKOM, J

HENRY, G

机构：

[1] Department of Molecular, Cellular and Developmental Biology, University of Colorado, Boulder

[2] Reproductive Genetics In Vitro, Denver

来源：

HUMAN REPRODUCTION | 1992年 / 7卷 / 03期

关键词：

HUMAN OOCYTE; ANEUPLOIDY; CYTOGENETICS; CYTOPLASMIC ORGANIZATION; ASSISTED REPRODUCTION;

D O I：

10.1093/oxfordjournals.humrep.a137655

中图分类号：

R71 [妇产科学];

学科分类号：

100211 ;

摘要：

The frequency of aneuploidy in 583 newly aspirated, uninseminated metaphase II-stage human oocytes which exhibited seven distinct forms of cytoplasmic dysmorphism [Van Blerkom (1990) J. Electron Microsc. Tech., 16, 324] after ovarian stimulation and ovulation induction was determined in the living state by DNA fluorescence followed by fixation and air-drying for karyotyping. The findings demonstrate that as many as half of the oocytes with dysmorphic phenotypes which arise early in meiotic maturation are aneuploid, with hypohaploidy predominant. In contrast, cytoplasmic defects which occur at or after metaphase I are associated with a relatively low frequency of aneuploidy (< 15%), which is comparable to that previously reported for human oocytes with a normal cytoplasmic appearance [Van Blerkom and Henry (1988) Hum. Reprod., 3, 777]. The aetiologies of aneuploidy in dysmorphic oocytes, as well as the clinical implications for oocyte selection in laboratory-assisted conception are discussed.

引用

页码：379 / 390

页数：12

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