PURIFICATION AND CHARACTERIZATION OF AN EXOPOLYGALACTURONASE PRODUCED BY FUSARIUM-OXYSPORUM F SP RADICIS-LYCOPERSICI

被引：8

作者：

VAZQUEZ, C ^{[1
]}

PATINO, B ^{[1
]}

MARTINEZ, MJ ^{[1
]}

机构：

[1] CSIC, CTR INVEST BIOL, UNIDAD MICROBIOL APLICADA, MADRID 6, SPAIN

来源：

FEMS MICROBIOLOGY LETTERS | 1993年 / 110卷 / 02期

关键词：

EXOPOLYGALACTURONASE; FUSARIUM-OXYSPORUM F SP RADICIS-LYCOPERSICI;

D O I：

10.1016/0378-1097(93)90465-E

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

An exopolygalacturonase produced by Fusarium oxysporum f. sp. radicis lycopersici, a fungus that produces root rot, was purified by gel filtration and ion exchange chromatography. It had a M(r) 68 K, a pH optimum of 5.6 and an optimum temperature of 60-degrees-C. This polygalacturonase was inhibited by calcium ions and had a K(m) of 0.64 mM using sodium polypectate as substrate. The exo mode of action of this enzyme was revealed by thin-layer chromatography of hydrolysed substrate.

引用

页码：191 / 196

页数：6

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