RAPID DIAGNOSIS OF APPLE PROLIFERATION MYCOPLASMA-LIKE ORGANISM USING A POLYMERASE CHAIN-REACTION PROCEDURE

被引：13

作者：

FIRRAO, G

GOBBI, E

LOCCI, R

机构：

[1] Dipartimento di Biologia Applicata Alia Difesa Delle Piante, Università di Udine, Udine, I-33100

来源：

PLANT PATHOLOGY | 1994年 / 43卷 / 04期

关键词：

D O I：

10.1111/j.1365-3059.1994.tb01605.x

中图分类号：

S3 [农学（农艺学）];

学科分类号：

0901 ;

摘要：

The rapid diagnosis of apple proliferation mycoplasma-like organism, using polymerase chain reaction amplification of part of the 16S rRNA gene is reported. Apple proliferation MLO can be detected in as little as 0.3 g of diseased tissue. Results compare favourably with those obtained by other PCR-based methods. Operational speed, sensitivity, specificity and ease of performance of the method, with particular reference to indexing purposes, are discussed.

引用

页码：669 / 674

页数：6

共 19 条

[1] DETECTION OF DNA OF PLANT PATHOGENIC MYCOPLASMALIKE ORGANISMS BY A POLYMERASE CHAIN-REACTION THAT AMPLIFIES A SEQUENCE OF THE 16S RNA GENE [J].