ENZYMATIC-ACTIVITY OF A DEVELOPMENTALLY-REGULATED MEMBER OF THE SIALYLTRANSFERASE FAMILY (STX) - EVIDENCE FOR ALPHA-2,8-SIALYLTRANSFERASE ACTIVITY TOWARD N-LINKED OLIGOSACCHARIDES

被引：104

作者：

KOJIMA, N ^{[1
]}

YOSHIDA, Y ^{[1
]}

KUROSAWA, N ^{[1
]}

LEE, YC ^{[1
]}

TSUJI, S ^{[1
]}

机构：

[1] RIKEN,INST PHYS & CHEM RES,FRONTIER RES PROGRAM,WAKO,SAITAMA 35101,JAPAN

来源：

FEBS LETTERS | 1995年 / 360卷 / 01期

关键词：

SIALYLTRANSFERASE; GLYCOPROTEIN; N-LINKED OLIGOSACCHARIDE; GLYCOSYLTRANSFERASE GENE; POLYSIALIC ACID; NEURAL CELL ADHESION MOLECULE;

D O I：

10.1016/0014-5793(95)00059-I

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

We have detected sialyltransferase activity of recombinant mouse STX, which was cloned from rat brain as a new member of the sialyltransferase family, but sialyltransferase activity of which had not been detected previously [Livingston and Paulson, J. Biol. Chem, (1993) 268, 11504-11507]. The activity of mouse STX was specific toward sialylated glycoproteins, N-Glycanase treatment and linkage-specific sialidase treatment of glycoproteins revealed that STX transfers sialic acids through alpha 2,8-linkages to only N-linked oligosaccharides of glycoproteins, However, polymerase activity for polysialic acid synthesis was not detected for this sialyltransferase. Since this alpha 2,8-sialyltransferase gene is highly restricted in fetal and newborn brain, it may be involved in the polysialylation of glycoproteins, especially of N-CAM.

引用

页码：1 / 4

页数：4

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